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和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

蔺兴遥 刘炳涛 马晓辉 张扬 陈卫强 金晓东

蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东. 和厚朴酚对非小细胞肺癌细胞的辐射增敏效应[J]. 原子核物理评论, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
引用本文: 蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东. 和厚朴酚对非小细胞肺癌细胞的辐射增敏效应[J]. 原子核物理评论, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
Citation: MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104

和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

doi: 10.11804/NuclPhysRev.36.01.104
基金项目: 国家自然科学基金资助项目(U1730133);甘肃省自然科学基金资助项目(17JRSRA310)
详细信息
    作者简介:

    蔺兴遥(1975-),男,甘肃靖远人,副教授,硕士,从事中药药理与毒理研究;Email:878104134@qq.com

    通讯作者: 金晓东,E-mail:jinxd@imocas.ac.cn
  • 中图分类号: R730.55;R96

Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells

Funds: National Natural Science Foundation of China (U1730133); Natural Science Foundation of Gansu Province (17JRSRA310)
  • 摘要: 研究了和厚朴酚(HNK)对非小细胞肺癌(NSCLC)细胞系A549和H1299对低线性能量转移(LET) X射线和高LET碳离子的辐射增敏效应。首先用CCK-8检测了HNK对A549和H1299细胞的生长抑制情况,发现20 μmol/L的HNK处理对细胞的生长抑制作用较弱。用该浓度HNK预处理细胞2 h后给予不同剂量X射线或碳离子的照射,克隆存活法检测细胞的辐射敏感性,Annexin-PI双染法检测细胞凋亡,γH2AX焦点法检测DNA的双链断裂(DSB)损伤。实验结果显示:与X射线相比,NSCLC细胞对碳离子更敏感,HNK预处理仅对碳离子照射有辐射增敏作用;与碳离子单独照射相比,HNK预处理联合碳离子照射诱导了更明显的细胞凋亡;在照射后24 h,HNK预处理联合碳离子照射引起的细胞γH2AX焦点阳性率维持在较高水平,而X射线照射没有这些效应。实验结果表明,HNK预处理抑制了NSCLC细胞DNA的DSB修复,诱导了细胞凋亡的发生,从而提高了细胞对碳离子的辐射敏感性。


    The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.
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出版历程
  • 收稿日期:  2018-12-14
  • 修回日期:  2019-02-02
  • 刊出日期:  2019-03-20

和厚朴酚对非小细胞肺癌细胞的辐射增敏效应

doi: 10.11804/NuclPhysRev.36.01.104
    基金项目:  国家自然科学基金资助项目(U1730133);甘肃省自然科学基金资助项目(17JRSRA310)
    作者简介:

    蔺兴遥(1975-),男,甘肃靖远人,副教授,硕士,从事中药药理与毒理研究;Email:878104134@qq.com

    通讯作者: 金晓东,E-mail:jinxd@imocas.ac.cn
  • 中图分类号: R730.55;R96

摘要: 研究了和厚朴酚(HNK)对非小细胞肺癌(NSCLC)细胞系A549和H1299对低线性能量转移(LET) X射线和高LET碳离子的辐射增敏效应。首先用CCK-8检测了HNK对A549和H1299细胞的生长抑制情况,发现20 μmol/L的HNK处理对细胞的生长抑制作用较弱。用该浓度HNK预处理细胞2 h后给予不同剂量X射线或碳离子的照射,克隆存活法检测细胞的辐射敏感性,Annexin-PI双染法检测细胞凋亡,γH2AX焦点法检测DNA的双链断裂(DSB)损伤。实验结果显示:与X射线相比,NSCLC细胞对碳离子更敏感,HNK预处理仅对碳离子照射有辐射增敏作用;与碳离子单独照射相比,HNK预处理联合碳离子照射诱导了更明显的细胞凋亡;在照射后24 h,HNK预处理联合碳离子照射引起的细胞γH2AX焦点阳性率维持在较高水平,而X射线照射没有这些效应。实验结果表明,HNK预处理抑制了NSCLC细胞DNA的DSB修复,诱导了细胞凋亡的发生,从而提高了细胞对碳离子的辐射敏感性。


The radiosensitizing effect of Honokiol (HNK) on non-small cell lung carcinoma (NSCLC) cell lines A549 and H1299 to low-linear energy transfer (LET) X-rays and high-LET carbon ions was investigated in this study. First, the inhibitory effects of HNK on the growth of A549 and H1299 cells were detected by CCK-8 assay, and 20 μmol/L HNK treatment was found to induce a growth inhibitory effect slightly in these two cell lines. Cells were pre-treated with HNK and then irradiated with X-rays and carbon ions of different doses. Cellular radiosensitivity, apoptosis and DNA damage were analyzed by clonogenic survival, Annexin-PI staining and γH2AX foci, respectively. The results showed the cells were more sensitive to carbon ion irradiation compared to X-rays and the radiosensitization of HNK was only observed after carbon ion irradiation. Furthermore, the co-treatment led to higher apoptosis rate 48 h after irradiation and increased the positive rate of γH2AX foci 24 h after irradiation in A549 and H1299 cells compared with those in the groups treated with carbon ion irradiation alone. These phenomena were not observed after X-ray irradiation. Our data suggest that the pre-treatment with HNK inhibited DNA DSB repair, induced apoptosis and then enhanced the cellular radiosensitivity to carbon ions in NSCLC cells.

English Abstract

蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东. 和厚朴酚对非小细胞肺癌细胞的辐射增敏效应[J]. 原子核物理评论, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
引用本文: 蔺兴遥, 刘炳涛, 马晓辉, 张扬, 陈卫强, 金晓东. 和厚朴酚对非小细胞肺癌细胞的辐射增敏效应[J]. 原子核物理评论, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
Citation: MING Xingyao, LIU Bingtao, MA Xiaohui, ZHANG Yang, CHEN Weiqiang, JIN Xiaodong. Radiosensitizing Effect of Honokiol on Non-small Cell Lung Carcinoma Cells[J]. Nuclear Physics Review, 2019, 36(1): 104-110. doi: 10.11804/NuclPhysRev.36.01.104
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