Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio
doi: 10.11804/NuclPhysRev.27.04.469
- Received Date: 1900-01-01
- Rev Recd Date: 1900-01-01
- Publish Date: 2010-12-20
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Key words:
- clustered DNA damage /
- heavy ion /
- proton /
- γ ray
Abstract: Based on the molecular model of conformation changes of plasmid DNA, the DNA damages induced by γ rays, proton and 7Li ion were studied and compared. In order to detect the clustered DNA damage, the irradiated plasmid DNA was digested with E Coli formamidopyrimidine DNA glycosylase (Fpg) and AP endonuclease III (Endo III) which convert the damaged bases and AP sites into singlestrand breaks (SSB), and then subjected to agarose gel electrophoresis. The results indicated that SSB, a certain level of clustered bases lesions and a tiny of doublestrand breaks (DSB)were induced by the observed 50—200 Gy of γ rays. The DNA breakage and clustered bases lesions induced by high LET proton and 7Li ion were much higher as compared with that induced by γ rays. Our data also showed that an obvious clustered DNA damage was induced by proton even at 10 Gy dose, and the effectiveness of DNA damage induction by proton was higher than that of 7Li ion.
Citation: | XU Hui-hui, SUI Li, LIU Xiao-dan, WANG Li, WANG Xiao, WANG Yu, XU Qin-zhi, ZHOU Ping-kun, #. Comparative Studies on Clustered DNA Damage Induced by Different LET Ionizing Radiatio[J]. Nuclear Physics Review, 2010, 27(4): 469-473. doi: 10.11804/NuclPhysRev.27.04.469 |