Screening and Breeding of High Yield Strain of Protein Feed Yeast and Optimization of Its Fermentation Process

Liang MA; Zeya DU; Xiang ZHOU; Jihong CHEN

doi:10.11804/NuclPhysRev.39.2022063

Volume 39 Issue 4

Dec. 2022

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Nuclear Physics Review > 2022 > 39(4): 512-518. > DOI: 10.11804/NuclPhysRev.39.2022063

Liang MA, Zeya DU, Xiang ZHOU, Jihong CHEN. Screening and Breeding of High Yield Strain of Protein Feed Yeast and Optimization of Its Fermentation Process[J]. Nuclear Physics Review, 2022, 39(4): 512-518. DOI: 10.11804/NuclPhysRev.39.2022063

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Screening and Breeding of High Yield Strain of Protein Feed Yeast and Optimization of Its Fermentation Process

Liang MA^1,,
Zeya DU³,
Xiang ZHOU^{1, 2, ,},
Jihong CHEN^{1, 2, 3, ,}

1.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 73000, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
Kejin Innovation Institute of Heavy Ion Beam Biological Industry, Baiyin 730900, Gansu, China

Funds: Inner Mongolia Autonomous Region Science & Technology Specific Projects(E039211SZ1)

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Corresponding author:
Xiang ZHOU, E-mail: syannovich@impcas.ac.cn

Jihong CHEN, E-mail: chjh@impcas.ac.cn
Received Date: September 02, 2021
Revised Date: November 15, 2021

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Abstract

Abstract

Protein content in feed is a key index for evaluating feed quality, However, recent reports of determine the protein content of feed on dietary for feeds tuffs investigated as potential sources of protein content (yeast, lactic acid bacteria, etc.) and protein content of the forage. The protein content of feed yeast strains directly determines the protein content of feed, so it becomes the key to obtain good feed yeast strains. In this study, a heavy ion ¹²C⁶⁺ beam with an energy of 80 MeV/u was used for irradiation mutagenesis and breeding of starter yeast NJ3236(protein content of 40.64%), and the strains were screened and rescreened to obtain 100G-2 strain with high protein content. The protein content of the 100G-2 strain increased by 10.08% compared with that of the strain NJ3236. Then response surface analysis was used to optimize the fermentation medium. The optimal ratio of medium showed that sweet sorghum juice 20.95 g/L, corn steep liquor 18.17 g/L and magnesium sulfate 1.60 g/L. Under this condition, the soluble protein concentration reached 1.381 mg/mL, which was 8.7% higher than that before optimization.
- heavy ion irradiation,
- feed yeast,
- sweet sorghum juice,
- response surface methodology

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