65279;Breeding Study of Hyper Citric Acid Strain by Progressive Heavy Ion Irradiation
doi: 10.11804/NuclPhysRev.30.04.483
- Received Date: 1900-01-01
- Rev Recd Date: 1900-01-01
- Publish Date: 2013-12-20
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Key words:
- heavy ion beam /
- irradiation /
- fermentation experiment /
- citric acid concentration /
Abstract: Heavy 12C6+ ion beams in various high doses were employed to irradiate H3001 strain for screening Aspergillus niger strain for hyper citric acid production. Three high-yield strains were obtained after shaker fermentation test. Among the three strains, the strain hw317 was implemented shaker fermentation for stability test and 10~100 L pilot fermentation tank for citric acid productive maximization. Acid-base neutralization method was applied to determinate the content of citric acid in fermented liquid. The results showed that: when the secondary heavy ion 12C6+ dose was 857.8 Gy, both of the fatality rate (94.5%) and the positive mutation rate (8%) were highest. Through the shaker fermentation tests and 10 ~ 100 L pilot fermentation test, one strain hw317 was screened and obtained for hyper citric acid production. Consequently, the final citric acid acidity can reach up to 19.2±0.2% with controlling fermentation cycle for 60 h.
Citation: | CHEN Jihong, HU Wei, LI Wenjian, LIU Jing, WANG Shuyang, WEI Zihao. 65279;Breeding Study of Hyper Citric Acid Strain by Progressive Heavy Ion Irradiation[J]. Nuclear Physics Review, 2013, 30(4): 483-487. doi: 10.11804/NuclPhysRev.30.04.483 |