Damage Effects of Pollen Nuclei of Pinus thunbergii Induced by Ion Beam Implantation
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Graphical Abstract
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Abstract
The damage of pollen nuclei of Pinus thunbergii induced directly by ion implantation were measured by the laser confocal microscopy technique and the singlecell gel electrophoresis test. The results showed that ion implantation caused the nuclei structure to be damaged， leading to the nuclei degradation. The results of statistical analysis showed that the damage grade of nuclei was very correlative with the ion dosage. The damage degree of DNA in the nuclei at the level of single cells was increased with the increase of the ion implantation dose.
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