HRS/IRR in E.coli K12 Implanted by Low Energy Ion Beam and Effect of recA Gene on HRS/IRR
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Graphical Abstract
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Abstract
The HRS/IRR in Escherichia coli had been investigated withE.coli K12 wild strain MG1655 irradiated by the 30 keV N+. The curve of the dosesurvival effect showed the 30 keV N+ ion could induce the HRS/IRR at the dose less than 10×1014 ions/cm2. Moreover, the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains, wild strain MG1655, LE392 (recA+) and DH5α(recA-). The results showed the IRR was disappeared in recA- DH5α strain, but the HRS/IRR appeared in wild strain MG1655 and recA+ LE392, which suggested that recA gene and the DNA damages & their repairs including recA gene were essential element in HRS/IRR inducement.
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