Abstract: The medical radioisotopes ⁹⁹Mo/^99mTc, ^64,67Cu, ⁶⁸Ge/⁶⁸Ga, ⁸²Sr/⁸²Rb, ²¹¹At, ²²⁵Ac have been attracting a lot of attention in nuclear medicine. In this paper, the yields and product distributions of the above medical radioisotopes were studied with FLUKA code. The results show that the yield growth trend becomes slower with the increase of beam energy. The yield increased with the increase of irradiation time and decreased with the increase of cooling time. The change mainly depends on the half-life of the medical radioisotopes. The incident beam produces medical radioisotopes within the effective range in the isotope target. The reasonable target thickness can optimize the specific activity value of the medical radioisotopes. Moreover, the kinds and yields of impurity nuclides also increased with the increase of the beam energy. The reasonable irradiation energy and irradiation time can reduce the complexity of subsequent medical radioisotopes separation and purification. This work provides important information for the accelerator produced medical radioisotopes by FLUKA calculation.