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微束技术在放射生物学中的应用

郭娜 杜广华 刘文静 郭金龙 吴汝群 陈昊

郭娜, 杜广华, 刘文静, 郭金龙, 吴汝群, 陈昊. 微束技术在放射生物学中的应用[J]. 原子核物理评论, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
引用本文: 郭娜, 杜广华, 刘文静, 郭金龙, 吴汝群, 陈昊. 微束技术在放射生物学中的应用[J]. 原子核物理评论, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
GUO Na, DU Guanghua, LIU Wenjing, GUO Jinlong, WU Ruqun, CHEN Hao. Microbeam Application in Radiation Biology[J]. Nuclear Physics Review, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
Citation: GUO Na, DU Guanghua, LIU Wenjing, GUO Jinlong, WU Ruqun, CHEN Hao. Microbeam Application in Radiation Biology[J]. Nuclear Physics Review, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471

微束技术在放射生物学中的应用

doi: 10.11804/NuclPhysRev.33.04.471
基金项目: 中国科学院百人计划资助项目(Y150310BRO);国家自然科学基金委员会资助项目(31200630,U1632271);科技部重大仪器专项(2012YQ03014204)
详细信息
    作者简介:

    郭娜(1988-),女,河北邯郸人,硕士研究生,从事重离子微束在线活细胞成像研究;E-mail:naguo@impcas.ac.cn

    通讯作者: 杜广华,E-mail:gh_du@impcas.ac.cn
  • 中图分类号: Q691

Microbeam Application in Radiation Biology

Funds: Hundred Talents Program of Chinese Academy of Sciences (Y150310BRO), National Natural Science Foundation of China(31200630, U1632271), Ministry of Science and Technology(2012YQ03014204)
  • 摘要: 微束装置可以为生命科学研究提供微米定位、剂量特定的电离辐射,在生物体内的电离辐射靶物质及其敏感度、靶物质的损伤及修复机制研究中具有独特的作用。概述了生物微束装置和实验技术的发展及其在低剂量辐射效应、旁观者效应、信号传导研究中的主要应用;介绍了中国科学院近代物理研究所(IMP)重离子微束装置,该装置可以提供能量7~ 80 MeV/u、传能线密度为30~ 3000 keV/μm的重离子微束,实现了活细胞辐照和在线观察、小鼠定位辐照的实验技术;利用IMP微束装置在重离子诱导旁效应实验、小鼠下丘脑重离子辐照效应和DNA损伤快速修复动态等方面取得了一些实验成果。


    The microbeam facility can provide micrometer scale localized and predefined ionizing radiation in the life science study, and the microbeam techniques play a unique role in determining the target substances of ionizing radiation, as well as in the study of radiation sensitivity, mechanisms of radiation damage response and repair. This paper summarizes the technical developments of biological microbeam facilities and their applications in the studies of low-dose radiation effect, bystander effect and cellular signaling. This paper also introduces the recent developments at the heavy-ion microbeam facility in the Institute of Modern Physics (IMP), which can provide heavy ion microbeam irradiation with energy of 7~80 MeV/u and LET of 30~3000 keV/μm. The facility can perform radiobiological irradiation and online investigation in living cells and mice, including bystander effect study, sleeping system influence after irradiation to mice hypothalamus and the recruitment dynamics of XRCC1 protein.
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出版历程
  • 收稿日期:  2015-12-18
  • 修回日期:  2016-01-20
  • 刊出日期:  2016-12-20

微束技术在放射生物学中的应用

doi: 10.11804/NuclPhysRev.33.04.471
    基金项目:  中国科学院百人计划资助项目(Y150310BRO);国家自然科学基金委员会资助项目(31200630,U1632271);科技部重大仪器专项(2012YQ03014204)
    作者简介:

    郭娜(1988-),女,河北邯郸人,硕士研究生,从事重离子微束在线活细胞成像研究;E-mail:naguo@impcas.ac.cn

    通讯作者: 杜广华,E-mail:gh_du@impcas.ac.cn
  • 中图分类号: Q691

摘要: 微束装置可以为生命科学研究提供微米定位、剂量特定的电离辐射,在生物体内的电离辐射靶物质及其敏感度、靶物质的损伤及修复机制研究中具有独特的作用。概述了生物微束装置和实验技术的发展及其在低剂量辐射效应、旁观者效应、信号传导研究中的主要应用;介绍了中国科学院近代物理研究所(IMP)重离子微束装置,该装置可以提供能量7~ 80 MeV/u、传能线密度为30~ 3000 keV/μm的重离子微束,实现了活细胞辐照和在线观察、小鼠定位辐照的实验技术;利用IMP微束装置在重离子诱导旁效应实验、小鼠下丘脑重离子辐照效应和DNA损伤快速修复动态等方面取得了一些实验成果。


The microbeam facility can provide micrometer scale localized and predefined ionizing radiation in the life science study, and the microbeam techniques play a unique role in determining the target substances of ionizing radiation, as well as in the study of radiation sensitivity, mechanisms of radiation damage response and repair. This paper summarizes the technical developments of biological microbeam facilities and their applications in the studies of low-dose radiation effect, bystander effect and cellular signaling. This paper also introduces the recent developments at the heavy-ion microbeam facility in the Institute of Modern Physics (IMP), which can provide heavy ion microbeam irradiation with energy of 7~80 MeV/u and LET of 30~3000 keV/μm. The facility can perform radiobiological irradiation and online investigation in living cells and mice, including bystander effect study, sleeping system influence after irradiation to mice hypothalamus and the recruitment dynamics of XRCC1 protein.

English Abstract

郭娜, 杜广华, 刘文静, 郭金龙, 吴汝群, 陈昊. 微束技术在放射生物学中的应用[J]. 原子核物理评论, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
引用本文: 郭娜, 杜广华, 刘文静, 郭金龙, 吴汝群, 陈昊. 微束技术在放射生物学中的应用[J]. 原子核物理评论, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
GUO Na, DU Guanghua, LIU Wenjing, GUO Jinlong, WU Ruqun, CHEN Hao. Microbeam Application in Radiation Biology[J]. Nuclear Physics Review, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
Citation: GUO Na, DU Guanghua, LIU Wenjing, GUO Jinlong, WU Ruqun, CHEN Hao. Microbeam Application in Radiation Biology[J]. Nuclear Physics Review, 2016, 33(4): 471-480. doi: 10.11804/NuclPhysRev.33.04.471
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