Abstract: In recent years, the cluster structure in nuclei has attracted a lot of attention. The goal of this work is to investigate the yield distribution of heavy ion collisions at Fermi energy and evaluate the \alpha cluster configurations in light nuclei. The ¹⁶O+¹⁶O reactions with four different initialization \alpha configurations (long chain, kite, square, tetrahedron)are simulated based on the Extended Quantum Molecular Dynamics (EQMD) transport model. The nuclei with different structures are investigated by observation of the multiplicity distribution of yields in nuclear reactions. It is found that the yields of free protons and ⁴He were affected by different cluster configurations, which indicates that ⁴He/proton can be used as a characterization of cluster structure. In addition, the \alpha cluster configuration information of ¹⁶O can be extracted from the outgoing \theta and \phi and the kinetic energy spectrum of of free protons and ⁴He.