摘要: 非生物逆境胁迫是影响植物生长的重要因素之一，植物耐盐研究是抗逆研究的一个重要组成部分。利用兰州重离子研究装置（HIRFL）产生能量为43.3 MeV/u的碳离子束辐射诱变拟南芥干种子，获得了稳定突变体#197，通过生理指标测定和实时荧光定量PCR技术对该突变材料进行盐胁迫相关研究，结果表明：#197在盐胁迫条件下萌发速率低于野生型，幼苗的绿胚率较野生型更高。基因表达方面，#197受到高盐胁迫后，P5CS1基因迅速转录，且表达水平显著高于野生型，而脯氨酸降解相关基因ProDH的表达量#197略低于野生型，该表达模式有利于脯氨酸的积累，从而缓解了#197受到的胁迫压力，使得DREB2A和RD29A的表达量低于同期野生型。


Abiotic stress can reduce the growth of plant heavily, and the research of salt tolerance of plant mutant is valuable to the study plant stress resistance. Dry seeds of Arabidopsis thaliana were irradiated by carbon ion beams with energy of 43.3 MeV/u provided by Heavy Ion Research Facility in Lanzhou (HIRFL). One stable mutant #197 was obtained from the progenies of the irradiated populations, the study of tolerance to salt stress of this mutant were carried out through physiological and gene transcription anlaysis. The results demonstrated:mutant #197 shown a relatively lower germination rate than wild type in salt stress conditions, meanwhile, the mutant acquired a higher ratio of green embryo than the wild type. In the molecular level, NaCl with high concentrations prompted the transcription of P5CS1 gene rapidly both in #197 muatant and the wide type, and the expression level in mutant was significantly higher than wild type. The expression of ProDH, related to proline degradation, in mutant #197 was slightly less than the wide type. Such expression patterns improved the accumulation of proline, and protect the #197 mutant from salt stress, which may lead to a lower expression of DREB2A and RD29A in mutant compared with wide type.