GANRA-5对X射线和12C6+离子辐射防护作用研究
Radioprotective Effects of GANRA-5 to X-rays and 12C6+
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摘要: 研究了一种新的恶唑酮类衍生物GANRA-5 对于人胚肺细胞MRC-5 的辐射防护作用。以MTT评价其对于细胞的毒性，以H2AX foci 形成法检测其对于辐照后细胞中双链断裂的影响，发现其对于受到X射线和12C6+离子照射的细胞具有较强的辐射防护作用，并进一步发现其能够显著清除辐照后细胞内的自由基。这些结果表明，GANRA-5 具有较低的细胞毒性，并能够通过清除自由基发挥较强的针对X射线和12C6+离子的辐射防护作用，有望开发为高效的辐射防护药物。The radio-protective effects on human embryonic lung fibroblast MRC-5 of an oxazolone derivative named GANRA-5 were studied. MTT test was used to evaluate the toxicity and H2AX foci assay was used to test the influence of GANRA-5 on the DSB (double-stranded break) formation after irradiation. It is found that GANRA-5 exhibited low toxicity and high radio-protective effects on MRC-5 cells against both X-ray and 12C6+ ion beam. It is also found that it can act as a scavenger of free radicals. These results suggest that GANRA-5 is a promising radioprotectant.Abstract: The radio-protective effects on human embryonic lung fibroblast MRC-5 of an oxazolone derivative named GANRA-5 were studied. MTT test was used to evaluate the toxicity and H2AX foci assay was used to test the influence of GANRA-5 on the DSB (double-stranded break) formation after irradiation. It is found that GANRA-5 exhibited low toxicity and high radio-protective effects on MRC-5 cells against both X-ray and 12C6+ ion beam. It is also found that it can act as a scavenger of free radicals. These results suggest that GANRA-5 is a promising radioprotectant.
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