12C6+离子辐照对苜蓿M1代低温胁迫存活率、过氧化氢酶及过氧化物酶活性的影响
Effects of Carbon Ion Irradiation on Survival Rate,Catalase and Peroxidase Activity of Alfalfa M1 under Low Temperature Stress
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摘要: 研究了不同剂量12C6+离子辐照对中兰1 号、BC-04-477、塔城3 种苜蓿M1代个体在低温胁迫下存活率、过氧化氢酶(CAT) 及过氧化物酶(POD) 活性的影响。在辐照剂量为400 Gy 时，中兰1 号低温胁迫组存活率、CAT活性比未辐照的对照分别提高了33.3%，56.3%，POD活性与未辐照的对照无差异；在辐照剂量为400 Gy 时，BC-04-477 低温胁迫组存活率、CAT及POD 活性比未辐照的对照组分别提高了33.3%，69.2%，5.1%；塔城在辐照剂量为800 Gy时，低温胁迫组的存活率、CAT及POD活性比未辐照的对照组分别提高了25%，26%，22.8%。以上结果表明，12C6+离子辐照可以提高中兰1号、BC-04-477、塔城的低温环境的存活能力，提高苜蓿抗寒性能。In this study, three kinds of alfalfa including Zhonglan 1, BC-04-477 and Ta Cheng were treated with different doses of 12C6+ (75 keV) heavy ion radiation, and then the influence of survival rate, catalase(CAT) and peroxidase(POD) activity of M1 with low temperature stress were tested. The results showed that under the condition of 400 Gy radiation dose , the survival rate and CAT activity of Zhonglan 1 under low temperature stress have increased by 33.3%, 56.3% respectively compared with those of the control group, while there was no difference in POD activity between those two groups. The survival rate, CAT and POD activity of BC-04-477 treated with low temperature have been improved by 33.3%, 69.2%, 5.1% respectively compared with those of the control group when the radiation dose was 400 Gy. Compared with those of the control group, the survival rate, CAT and POD activity of Ta Cheng under low temperature stress have been improved by 25%, 26%,22.8% respectively when the radiation dose was 800 Gy. These results indicate that the viability and the cold resistance ability of Zhong Lan 1, BC-04-477 and Ta Cheng can be improved by 12C6+ radiation.Abstract: In this study, three kinds of alfalfa including Zhonglan 1, BC-04-477 and Ta Cheng were treated with different doses of 12C6+ (75 keV) heavy ion radiation, and then the influence of survival rate, catalase(CAT) and peroxidase(POD) activity of M1 with low temperature stress were tested. The results showed that under the condition of 400 Gy radiation dose , the survival rate and CAT activity of Zhonglan 1 under low temperature stress have increased by 33.3%, 56.3% respectively compared with those of the control group, while there was no difference in POD activity between those two groups. The survival rate, CAT and POD activity of BC-04-477 treated with low temperature have been improved by 33.3%, 69.2%, 5.1% respectively compared with those of the control group when the radiation dose was 400 Gy. Compared with those of the control group, the survival rate, CAT and POD activity of Ta Cheng under low temperature stress have been improved by 25%, 26%,22.8% respectively when the radiation dose was 800 Gy. These results indicate that the viability and the cold resistance ability of Zhong Lan 1, BC-04-477 and Ta Cheng can be improved by 12C6+ radiation.
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