低能N离子注入对蚕豆根尖细胞的诱变效应
Effects of Irradiation with Low energy Nitrogen Ion Injection on Root Tip Cells of Broad Bean
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摘要: 为了研究低能N离子束的细胞遗传学效应， 以不同剂量的N离子束对蚕豆种子的种胚进行辐照， 观察分析根尖细胞的微核率、 有丝分裂指数和染色体畸变效应。 研究发现， 离子束的注入抑制了根尖细胞的有丝分裂， 干扰了正常的有丝分裂过程， 引发了染色体的结构、 行为和数目畸变； 随着离子注入剂量的增加， 微核率增加、 有丝分裂指数降低、 染色体畸变率增加。 In order to study the cytogenetic effects of lowenergy nitrogen ion irradiation, broad bean seed embryo was irradiated by different doses of nitrogen ions. Micronucleus rate, mitotic index and chromosome aberration in roottip cells were analyzed. The results showed that the injection of ions inhibited mitosis of root tip cells, interfered the normal process of mitosis, caused aberrations of chromosome structure, behavior and number. The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage, while mitotic index decreased.Abstract: In order to study the cytogenetic effects of lowenergy nitrogen ion irradiation, broad bean seed embryo was irradiated by different doses of nitrogen ions. Micronucleus rate, mitotic index and chromosome aberration in roottip cells were analyzed. The results showed that the injection of ions inhibited mitosis of root tip cells, interfered the normal process of mitosis, caused aberrations of chromosome structure, behavior and number. The frequency of micronucleus and chromosomal aberrations increased with the increasing radiation dosage, while mitotic index decreased.
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