12C6+离子对阿维链霉菌累进辐照诱变效应研究
Mutagenic Effect of Streptomyces Avermitilis Progressivity Irradiated by Ion Beam of 12C6+
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摘要: 选用12C6+ 离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变, 研究其累进辐照效应。实验结果表明,在辐照剂量为10 Gy时, 原始菌株比诱变高产菌株存活率高, 抗辐射能力强;辐照剂量高于30 Gy时,诱变高产菌株比原始菌株存活率高, 抗辐射能力强。原始菌株正突变率最高的辐照剂量为50 Gy, 致死率99.43%,正突变率最高, 达34.2%;对诱变高产菌株辐照剂量为30 Gy,致死率94.97%,正突变率最高, 达23.5% 。累进辐照效应降低了最佳辐照剂量。 Mutagenic effect on the mutant highproducing strain ZJAVY1203 and the original strain ZJAVA1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant highproducing Strain at dose of 10 Gy. The mutant highproducing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAVY1203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.
Abstract: Mutagenic effect on the mutant highproducing strain ZJAVY1203 and the original strain ZJAVA1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant highproducing Strain at dose of 10 Gy. The mutant highproducing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAVY1203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.