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12C6+离子对阿维链霉菌累进辐照诱变效应研究

Mutagenic Effect of Streptomyces Avermitilis Progressivity Irradiated by Ion Beam of 12C6+

  • 摘要: 选用12C6+ 离子束对阿维链霉菌诱变选育高产菌株与原始菌株进行辐照诱变, 研究其累进辐照效应。实验结果表明,在辐照剂量为10 Gy时, 原始菌株比诱变高产菌株存活率高, 抗辐射能力强;辐照剂量高于30 Gy时,诱变高产菌株比原始菌株存活率高, 抗辐射能力强。原始菌株正突变率最高的辐照剂量为50 Gy, 致死率99.43%,正突变率最高, 达34.2%;对诱变高产菌株辐照剂量为30 Gy,致死率94.97%,正突变率最高, 达23.5% 。累进辐照效应降低了最佳辐照剂量。 Mutagenic effect on the mutant highproducing strain ZJAVY1203 and the original strain ZJAVA1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant highproducing Strain at dose of 10 Gy. The mutant highproducing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAVY1203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.

     

    Abstract: Mutagenic effect on the mutant highproducing strain ZJAVY1203 and the original strain ZJAVA1 irradiated by ion beam of 12C6+ have been investigated. The experimental results indicated that the original strain has higher survival rate and stronger resistance to radiation than mutant highproducing Strain at dose of 10 Gy. The mutant highproducing strain has higher survival rate and stronger resistance to radiation than the original strain at the dose higher than 30 Gy. The lethality was 97% and the highest rate of orthomutation was 34.2%, when ZJAV A1 was irradiated by 50 Gy 12C6+ beam. The lethality was 94.97% and the highest rate of orthomutation was 23.5% when ZJAVY1203 was irradiated by 30 Gy 12C6+ beam. The best radiation dose is decreased by progressivity irradiation.

     

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