12C6+高能重离子辐照大葱损伤及其分子生物学效应
Cytological Damage and Molecular Biology Effect of 12C6+ Heavy Ions on Allium fistulosum L.
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摘要: 利用30， 90， 180 Gy 3种剂量的12C6+重离子束辐照大葱种子， 研究其在细胞水平和农艺性状的诱变效应并进行RAPD分析。通过与M1代的研究结果比较后表明: 经过不同剂量12C6+重离子照射后能有效地诱导大葱细胞形成微核和染色体畸变， 这种诱变效应， 在M2代仍然有所表现。M1代大葱结果期的株高、 白长、 花序直径和种子产量随辐照剂量增加产生明显差别， 其中30 Gy辐照组增幅最大。大葱总水溶性蛋白质和维生素C的含量在30 Gy组中积累最多， 在90 Gy组有明显下降。与M1代一致， M2代中大葱染色体微核率及RAPD分析所得的DNA多态性比率仍然与辐照剂量呈正相关， 但比率整体下降； 说明高能量重离子辐照造成的DNA变异在M2代被修复和淘汰。
Abstract： The Allium fistulosum L. seeds were irradiated by 12C6+ heavy ions to the dosages of 30, 90, 180 Gy, the mutagenic effect in the aspect of the cell level and the agronomy was studied and the RAPD analysis was carried out. Comparision with the conclusion of M1 gengeration indicates that the Cytological damage, micronucleus and chromosomal aberration caused by radiation formed in the cells of Allium fistulosum L., and this kind of effect still existed in the M2 generation. There is a negative correlation between some of the growth indexes such as plant height, diameter of onion white and irradiation dosage to a certain extent, the growth indexes in the 30 Gy dosage exposure group are better than those in control group. The Allium fistulosum L. nutrients, including the total watersoluble protein and the Vitamin C content are the highest for the 30 Gy group and the lowest in 90 Gy group. Consistent with the M1 generation, the chromosomal aberrations, micronucleus and the DNA polymorphism rate by RAPD analysis are still positive correlations with the radiation dose in M2 generation respectively. However, the overall rates decline. The result indicated that the DNA variation induced by the high energy heavy ion exposure is repaired and eliminated to a certain extent in the M2 generation.Abstract:
Abstract： The Allium fistulosum L. seeds were irradiated by 12C6+ heavy ions to the dosages of 30, 90, 180 Gy, the mutagenic effect in the aspect of the cell level and the agronomy was studied and the RAPD analysis was carried out. Comparision with the conclusion of M1 gengeration indicates that the Cytological damage, micronucleus and chromosomal aberration caused by radiation formed in the cells of Allium fistulosum L., and this kind of effect still existed in the M2 generation. There is a negative correlation between some of the growth indexes such as plant height, diameter of onion white and irradiation dosage to a certain extent, the growth indexes in the 30 Gy dosage exposure group are better than those in control group. The Allium fistulosum L. nutrients, including the total watersoluble protein and the Vitamin C content are the highest for the 30 Gy group and the lowest in 90 Gy group. Consistent with the M1 generation, the chromosomal aberrations, micronucleus and the DNA polymorphism rate by RAPD analysis are still positive correlations with the radiation dose in M2 generation respectively. However, the overall rates decline. The result indicated that the DNA variation induced by the high energy heavy ion exposure is repaired and eliminated to a certain extent in the M2 generation.
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