阿佛曼链霉菌的12C离子辐照效应及高产菌株选育
Research on Mutation Breeding of Highproducing Strains from Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+
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摘要: 选用12C6+离子辐照诱变阿维菌素B1a产生菌ZJAV-A1， 研究其诱变效应。 实验结果表明， 12C6+离子辐照剂量50 Gy时致死率97%，正突变率最高可达到34.2%。 通过12C6+离子诱变处理，结合平板培养基及斜面培养基的正突变菌株筛选， 最终获得一株稳定性良好， 阿维菌素B1a组分产量稳定在4460—4588 μg/ml之间， 较出发菌株提高11.1%—14.7%的突变株ZJAVY1203。 Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of 12C6+ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy 12C6+. After the mutagenesis processing by ion beam of 12C6+ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.Abstract: Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of 12C6+ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy 12C6+. After the mutagenesis processing by ion beam of 12C6+ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.
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