低能离子注入E.coli K12的 HRS/IRR效应及recA 基因在其诱发中的作用
HRS/IRR in E.coli K12 Implanted by Low Energy Ion Beam and Effect of recA Gene on HRS/IRR
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摘要: 以MG1655(野生型), LE392(recA-)和DH5α(recA-)3株E.coliK12菌株为材料, 研究了30 keV N+离子注入E.coli K12时HRS/IRR效应的诱发情况及recA 基因在其诱发中的作用。 结果显示: 小于10×1014ions/cm2低剂量离子注入大肠杆菌可诱发HRS/IRR效应; 30 keV N+离子注入MG1655, LE392菌株都可诱发HRS/IRR效应, 而在DH5α菌株中无法诱导 IRR效应。 recA-与HRS/IRR 效应相斥性表明recA 基因在HRS/IRR效应的诱发中发挥了重要作用。 The HRS/IRR in Escherichia coli had been investigated withE.coli K12 wild strain MG1655 irradiated by the 30 keV N+. The curve of the dosesurvival effect showed the 30 keV N+ ion could induce the HRS/IRR at the dose less than 10×1014 ions/cm2. Moreover, the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains, wild strain MG1655, LE392 (recA+) and DH5α(recA-). The results showed the IRR was disappeared in recA- DH5α strain, but the HRS/IRR appeared in wild strain MG1655 and recA+ LE392, which suggested that recA gene and the DNA damages & their repairs including recA gene were essential element in HRS/IRR inducement.Abstract: The HRS/IRR in Escherichia coli had been investigated withE.coli K12 wild strain MG1655 irradiated by the 30 keV N+. The curve of the dosesurvival effect showed the 30 keV N+ ion could induce the HRS/IRR at the dose less than 10×1014 ions/cm2. Moreover, the effect of recA gene in HRS/IRR inducement had been researched with three E.coli K12 strains, wild strain MG1655, LE392 (recA+) and DH5α(recA-). The results showed the IRR was disappeared in recA- DH5α strain, but the HRS/IRR appeared in wild strain MG1655 and recA+ LE392, which suggested that recA gene and the DNA damages & their repairs including recA gene were essential element in HRS/IRR inducement.