真空、低能离子注入对人宫颈癌（HeLa）细胞中 p53及c-fos mRNA的表达影响

摘要: 在具有高效护水功能石蜡油的保护下进行了人宫颈癌细胞(HeLa细胞)的低能离子注入及真空处理，用荧光定量PCR的方法分别研究了真空和30 keV N⁺的不同注入剂量下，细胞中p53基因和c-fos基因的mRNA表达变化。结果表明, 上述两个基因的表达与真空及低能离子注入之间存在着剂量关系。

Abstract: The relationship between gene expression and lowenergy ion implantation has been studied. Because vacuum is prerequisite in lowenergy ion implantation， mineral oil was used to protect cells from water evaporating. In this study， HeLa cells were implanted by lowenergy ions (30 keV N⁺) at different doses, and p53 gene and c fos gene were studied with realtime quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×1014 ions/cm².