hprt基因突变分析方法及其在辐射生物学中的应用

Progress in hprt Mutation Assay and Its Application in Radiation Biology

摘要: hprt基因（次黄嘌呤鸟嘌呤磷酸核糖转移酶基因）由于其本身所具有的独特生物学特性，逐渐成为基因突变机制和修复机制理想的研究靶点， hprt基因突变分析法也逐渐成为很有价值并被广泛应用的生物剂量计。系统地综述了hprt基因的生物学特性、突变的检测方法学及其在辐射事故分析、放射治疗和宇航事业研究中的应用和进展。

Abstract: hprt gene is an Xlinked locus that has been well studied and widely used as a biomarker in mutation detection. hprt mutation assay is a gene mutation test system in mammalian cells in vitro which has been used as a biological dosimeter. In this paper， the biological characteristics of hprt gene， hprt mutation detection methodology and the application of hprt mutation assay in radiation biology are comprehensively reviewed.