重离子辐照对人肝癌HepG2细胞蛋白质组图谱的影响
Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells
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摘要: 分别制备经1 Gy C离子辐照和未经辐照人肝癌HepG2细胞的总蛋白样品， 采用固相pH梯度双向凝胶电泳技术进行蛋白分离， 用ImageMaster 2D双向电泳凝胶图像分析软件分析数字化图谱。 结果显示, 差异表达的蛋白质点为17个， 其中1个仅在未经C离子辐照的HepG2细胞中表达， 8个蛋白质点在辐照后的细胞中表达上调， 8个蛋白质点在辐照后的细胞中表达下调。 建立了重复性较好和分辨率较高的分离HepG2细胞总蛋白的双向电泳技术。 实验结果表明, C离子辐照HepG2细胞后其蛋白质组发生了改变。Abstract: To gain a better understanding of the molecular basis of the biological effects induced by heavy ion irradiation， total proteins extracted from human hepatoma HepG2 cells that unirradiated and irradiated with an intermediateenergy carbon ion beam at 1 Gy were separated by twodimensional gel electrophoresis (2DE) with immobilized pH gradients (IPGs). The 2DE profiles were analyzed with the ImageMaster 2D software. More than 500 proteins spots were reproducibly detected on each gel and 17 were differentially expressed for the unirradiated and irradiated cells. 1 protein was expressed only in the unirradiated cells. 8 proteins were downregulated and 8 proteins were upregulated after the carbon ion irradiation. In this study， reproducible 2DE profiles were established and some differential expressed proteins were detected. These basic data are very useful for the further study of heavyion biological effect.
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