质子照射小鼠组织病理损伤的初步观察
Initiatory Pathologic Observation of Protonirradiated Mice’s Organs
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摘要: 利用HI-13串列加速器提供的19 MeV 质子照射麻醉小鼠2, 4, 8和16 Gy ， 照射后不同时间麻醉处死小鼠进行病理组织学观察。 结果显示， 照射后各组小鼠腰背部开始环状脱毛， 病理改变为表皮细胞坏死、 脱落， 皮下水肿， 心、 肝出现小灶性坏死或伴出血， 肝细胞明显再生， 肺组织可出现充血及肺泡间隔明显增厚， 肾小球出现明显固缩、 坏死及消失。 这些病理改变在大剂量照射组尤为明显。 通过观察质子照射小鼠组织病理损伤效应， 为质子损伤的防治研究提供参考依据。Space radiation come from galactic cosmic rays and solar particle events which contain considerable protons is an invisible killer to astronauts. Furthermore， many wounded persons were injured by proton and other heavy ion radiation in a nuclear warhead or nuclear terrorism attack. However， the damage effect of proton radiation is known little in our country. In this study， the proton induced histopathologic changes of mice were observed primarily. naesthetic mice were irradiated with the 19 MeV protons dili vered by HI13 tandem accelerator at CIAE in different doses(2， 4， 8， 16 Gy). In order to observe the da mage effect， mice were killed by anaesthetic at different time point after radiation， and the tissue sections were analyzed by a histopathologic method. The results show that all of the mice’s backs began displaying ringed depilation at postirradiation day 7. Many epidermal cells necrosis and exfoliation happened， and subcutaneous dropsy was observed. There were some focal necrosis or even followed with bleeding in heart and liver. Hepatocyte evidently regenerated. A hyperaemia was seen in lung tissue and alveolar septum was obviously thickening. Acinus renis represented notable pyknosis， necrosis and disappearance. Especially， these pathologic alteration clearly displayed in high dose groups. In conclusion， protonradiation can induce different grade injury to skin， heart， liver， lung， kidney and other organs. Further studies should be made to deeply understand the mechanism about the proton radiation damage.
Abstract: Space radiation come from galactic cosmic rays and solar particle events which contain considerable protons is an invisible killer to astronauts. Furthermore， many wounded persons were injured by proton and other heavy ion radiation in a nuclear warhead or nuclear terrorism attack. However， the damage effect of proton radiation is known little in our country. In this study， the proton induced histopathologic changes of mice were observed primarily. naesthetic mice were irradiated with the 19 MeV protons dili vered by HI13 tandem accelerator at CIAE in different doses(2， 4， 8， 16 Gy). In order to observe the da mage effect， mice were killed by anaesthetic at different time point after radiation， and the tissue sections were analyzed by a histopathologic method. The results show that all of the mice’s backs began displaying ringed depilation at postirradiation day 7. Many epidermal cells necrosis and exfoliation happened， and subcutaneous dropsy was observed. There were some focal necrosis or even followed with bleeding in heart and liver. Hepatocyte evidently regenerated. A hyperaemia was seen in lung tissue and alveolar septum was obviously thickening. Acinus renis represented notable pyknosis， necrosis and disappearance. Especially， these pathologic alteration clearly displayed in high dose groups. In conclusion， protonradiation can induce different grade injury to skin， heart， liver， lung， kidney and other organs. Further studies should be made to deeply understand the mechanism about the proton radiation damage.
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