生物物理新技术在ATP合成酶超分子结构中的应用
Applications of New Biophysical Techniques to Supramolecular Structure of ATP Synthase
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摘要: 现代物理技术与方法的发展为腺苷三磷酸(ATP)合成酶分子的研究提供了丰富而有效的手段。 介绍了ATP合成酶研究中常用的物理技术与方法如质谱技术、 核磁共振技术、 X射线衍射技术、 红外光谱和紫外光谱技术的物理原理及其在ATP合成酶研究中的应用， 并重点介绍了新兴非常规手段如原子力显微镜、 荧光共振能量转移技术在ATP合成酶研究中的最新研究成果； 通过对比诸多技术与方法近年来在国内外研究中的进展情况， 对各种技术与方法的优缺点进行了阐述。
Abstract: The developing modern physical techniques offer a series of abundant and effective methods to study ATP synthase in structure and function. Firstly we stressed on the dialectic relationship between physical techniques and the improvement of science in history, and introduced a lot of physical techniques in common use in protein researches such as mass spectroscopy， nuclear magnetic resonance， synchronization Xray diffraction， infrared spectroscopy and ultraviolet spectroscopy, and then reviewed their application status in quo to ATP synthase. Secondly we paid our attention to the burgeoning unconventionally instruments, i.e., the atomic force microscope and the fluorescence resonance energy transform (FRET) which have attracted the professional attention， and introduced latest application and researches’ achievements. Compared the development of the techniques in recent years， we have set forth the shortcoming and excellence of all kinds of equipments introduced. And it was ended with the conclusion that it is necessary to manage the possible instruments effectively and sufficient for the personalities， and given out the optimum research routes which emphasized on the new techniques and novel methods, i.e., the atomic force microscope and FRET.
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