重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

段昕; 张红; 邱嵘; 高清祥; 闵凤玲; 郝冀芳; 刘兵; 周清明; 李小达; 王燕玲; 周光明

doi:10.11804/NuclPhysRev.24.01.055

重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

摘要

摘要: 探讨¹²C⁶⁺ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMVGFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。采用不同剂量的¹²C⁶⁺ 重离子束辐射经AdCMVGFP 转染的B16细胞，利用流式细胞仪检测腺病毒的转染率。结果表明， ¹²C^6＋重离子束辐射能提高腺病毒对B16细胞的转染率，且具有量效关系。此外，先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.

Abstract: The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.

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