人肝癌SMMC-7721细胞对低剂量γ射线辐射超敏感性的研究

Study on Hyper-radiosensitivity Effect of Human Hepatoma SMMC-7721 Cells Exposed to Low Dose γ-rays

摘要: 研究了人肝癌细胞SMMC-7721在低剂量γ射线照射下超敏感性和增强的辐射抗性响应。选用对数生长期细胞接受0—6 Gy不同剂量的^60Coγ射线的照射。利用流式细胞仪对细胞进行分选计数，并用克隆形成法检测细胞存活率。发现SMMC-7721细胞存在低剂量辐射超敏感性和增强的辐射抗性响应，即在0—0．3Gy之间细胞表现出单位剂量杀伤增强现象，在0．3—1Gy细胞表现一定的辐射抗性，在1Gy以上，细胞的存活符合线性平方模型。

Abstract: The low-dose responses such as hypersensitivity and increased radio-resistance of human hepatoma SMMC-7721 cells were studied in this work. Exponentially growing SMMC-7721 cells were irradiated with ~(60)Co γ-rays at doses of 0—6 Gy. Together with flow cytometer for precise cell sorting, cell survival fractions were measured by means of conventional colony-formation assay. It has been found that the SMMC-7721 cells showed hyper-radiosensitivity response at low doses and increased radio-resistance at larger