重离子辐照玉米种子M1代诱变效应研究
Study of M1 Mutagenic Effects of Heavy Ion Irradiation on Maize Seeds
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摘要: 用重离子^7Li和^12C以及^60Co的γ射线分别辐照玉米种子胚，研究重离子束辐照玉米种子M1代的生物学效应．结果表明：^60Co的丫射线使种子的发芽势和发芽率降低，重离子^7Li和^12C对发芽势和发芽率的抑制作用不明显；重离子辐照后，单株之间光合速率差异增大；M1代植株在雄性育性、叶片颜色及株高等方面发生了多种变化，并产生了一株双胚苗．应用随机多态性DNA分析技术，发现双胚苗的大、小苗之间及其与亲本自交系478在随机多态性DNA指纹上存在的差异，从分子水平上初步证实了双胚苗为自交系478的变异类型．In order to study M_(1) biological effects of heavy ion irradiation on maize seeds, the embryos of dry maize seeds are irradiated with~(7)Li ions and~(12)C ions as well as ~( 60)Co gamma rays. The results are as follows: ~( 60)Co gamma rays decrease germination impetus and germination rate of maize seeds, while the inhibitory effects of germination impetus and germination rate induced by the heavy ions~(7)Li and~(12)C are not significant. The difference of photosynthetic rates among the plants irradiate...Abstract: In order to study M_(1) biological effects of heavy ion irradiation on maize seeds, the embryos of dry maize seeds are irradiated with~(7)Li ions and~(12)C ions as well as ~( 60)Co gamma rays. The results are as follows: ~( 60)Co gamma rays decrease germination impetus and germination rate of maize seeds, while the inhibitory effects of germination impetus and germination rate induced by the heavy ions~(7)Li and~(12)C are not significant. The difference of photosynthetic rates among the plants irradiate...
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