Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation

CUI Da-chao; LI Qiang; LIU Xin-guo; DAI Zhong-ying

doi:10.11804/NuclPhysRev.29.02.189

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Article Navigation > Nuclear Physics Review > 2012 > 29(2): 189-195

CUI Da-chao, LI Qiang, LIU Xin-guo, DAI Zhong-ying, . Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation[J]. Nuclear Physics Review, 2012, 29(2): 189-195. doi: 10.11804/NuclPhysRev.29.02.189

Citation:

CUI Da-chao, LI Qiang, LIU Xin-guo, DAI Zhong-ying, . Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation[J]. Nuclear Physics Review, 2012, 29(2): 189-195. doi: 10.11804/NuclPhysRev.29.02.189

Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation

doi: 10.11804/NuclPhysRev.29.02.189

1.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2.Key Laboratory of Heavy Ion Radiation Biology and Medicineof Chinese Academy of Sciences, Lanzhou 730000, China;3.Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2012-06-20

Abstract

Signaling factors usually play an important role in bystander effect. In this work， human hepatoma QGY7703 cells in vitro were irradiated with highLET carbon ions. Concentrations of signaling factors such as TGF-β1 and NO were measured in the media of the irradiated QGY-7703 cells at different time points after irradiation. The conditioned media harvested at various times postirradiation were transferred to human hepatocyte L02 cells as bystander cells and then the influence of the conditioned media on survival fraction and cell viability of the bystander cells were determined. The results show that the irradiated cells regulate the concentration of the signaling factors released nearby themselves temporally and spatially， and the bystander cells response to the signaling factors differentially according to the concentration change. This work provides new basic data for exploring the bystander effect， especially caused by highLET radiation.
- bystander effect,
- highLET radiation,
- TGF-β1,
- NO,
- signaling factor
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation

doi: 10.11804/NuclPhysRev.29.02.189

1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2.Key Laboratory of Heavy Ion Radiation Biology and Medicineof Chinese Academy of Sciences, Lanzhou 730000, China;3.Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2012-06-20

Key words:

Abstract: Signaling factors usually play an important role in bystander effect. In this work， human hepatoma QGY7703 cells in vitro were irradiated with highLET carbon ions. Concentrations of signaling factors such as TGF-β1 and NO were measured in the media of the irradiated QGY-7703 cells at different time points after irradiation. The conditioned media harvested at various times postirradiation were transferred to human hepatocyte L02 cells as bystander cells and then the influence of the conditioned media on survival fraction and cell viability of the bystander cells were determined. The results show that the irradiated cells regulate the concentration of the signaling factors released nearby themselves temporally and spatially， and the bystander cells response to the signaling factors differentially according to the concentration change. This work provides new basic data for exploring the bystander effect， especially caused by highLET radiation.

Citation:

CUI Da-chao, LI Qiang, LIU Xin-guo, DAI Zhong-ying, . Concentration of Signaling Factors Released by Cells Irradiated with Carbon Ions at Different Time Points Postirradiation[J]. Nuclear Physics Review, 2012, 29(2): 189-195. doi: 10.11804/NuclPhysRev.29.02.189