The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding

DU Yan; YU Li-xia; LIU Qing-fang; ZHOU Li-bin; #; LI Wen-jian; #

doi:10.11804/NuclPhysRev.28.03.343

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Article Navigation > Nuclear Physics Review > 2011 > 28(3): 343-348

DU Yan, YU Li-xia, LIU Qing-fang, ZHOU Li-bin, #, LI Wen-jian, #. The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding[J]. Nuclear Physics Review, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

Citation:

DU Yan, YU Li-xia, LIU Qing-fang, ZHOU Li-bin, #, LI Wen-jian, #. The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding[J]. Nuclear Physics Review, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding

doi: 10.11804/NuclPhysRev.28.03.343

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2011-09-20

Abstract

To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by ¹²C⁶⁺heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to ¹²C⁶⁺heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.
- The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding,
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding

doi: 10.11804/NuclPhysRev.28.03.343

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2011-09-20

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The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding /
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Abstract: To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by ¹²C⁶⁺heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to ¹²C⁶⁺heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.

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