Effects of Irradiation with ¹²C⁶⁺ Ions on M1 Characters of Perilla frutescens(L.) Britt

Key words: 
• Perilla frutescens (L.) Britt /
• ¹²C⁶⁺ ion beam /
• mutation breeding /
• biological character /
• chromosomal aberration

Abstract: To investigate the M1 biological effects of heavy ions on Perilla frutescens (L.), its dry seeds of it were irradiated by ¹²C⁶⁺ with the dose of 0, 40, 80 or 120 Gy respectively at the rate of 4 Gy/min delivered by the Heavy Ion Research Facility in Lanzhou(HIRFL). The results showed that biological characters such as germinating rate, germinating potentiality， survival rate， plant height， numbers of branch， yield per plant and 1000grain weight were changed after irradiation. Moreover, the germinating potentia lity, yield per plant and 1000grain weight decreased in a dosedependent manner. The germinating rate， survival rate， plant height and numbers of branch exhibited obviously “parabola” trends with irradiation doses. The frequency of micronucleus and chromosomal aberration in roottip cells of Perilla frutescens (L.) was observed “linear rise” trend with the increase of radiation dosage. Data suggest that exposure with lowdose 12C6+ to seeds of Perilla frutescens (L.) has obvious injury effects at the first generation， and the lowdose irradiation has apparent promotive function to the germinating rates and survival rates in the range of irradiation dosage at this experiment.