金纳米粒子对黑色素瘤细胞的重离子辐射增敏效应研究

张鹏程; 刘岩; 金晓东; 陈卫强; 李强

doi:10.11804/NuclPhysRev.36.04.492

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金纳米粒子对黑色素瘤细胞的重离子辐射增敏效应研究

张鹏程, 刘岩, 金晓东, 陈卫强, 李强

文章导航 > 原子核物理评论 > 2019 > 36(4): 492-498

张鹏程, 刘岩, 金晓东, 陈卫强, 李强. 金纳米粒子对黑色素瘤细胞的重离子辐射增敏效应研究[J]. 原子核物理评论, 2019, 36(4): 492-498. doi: 10.11804/NuclPhysRev.36.04.492

引用本文:

ZHANG Pengcheng, LIU Yan, JIN Xiaodong, CHEN Weiqiang, LI Qiang. Radiosensitizing Effect of Gold Nanoparticles on Melanoma Cells Under Heavy Ion Irradiation[J]. Nuclear Physics Review, 2019, 36(4): 492-498. doi: 10.11804/NuclPhysRev.36.04.492

Citation:

ZHANG Pengcheng, LIU Yan, JIN Xiaodong, CHEN Weiqiang, LI Qiang. Radiosensitizing Effect of Gold Nanoparticles on Melanoma Cells Under Heavy Ion Irradiation[J]. Nuclear Physics Review, 2019, 36(4): 492-498. doi: 10.11804/NuclPhysRev.36.04.492

金纳米粒子对黑色素瘤细胞的重离子辐射增敏效应研究

doi: 10.11804/NuclPhysRev.36.04.492

张鹏程^1,2,3,4,
刘岩^1,2,3,4,
金晓东^1,2,3,4,
陈卫强^1,2,3,4,
李强^1,2,3,4,

1.
中国科学院近代物理研究所, 兰州 730000;
2.
中国科学院重离子束辐射生物医学重点实验室, 兰州 730000;
3.
甘肃省重离子束辐射医学应用基础重点实验室, 兰州 730000;
4.
中国科学院大学核科学与技术学院, 北京 100049

基金项目: 科技部重点研发计划资助项目（2018YFC0115700，2018YFC0115702）；甘肃省重大科技专项项目（1602FKDA005）；国家自然科学基金资助项目（11875299，11805247）

详细信息

作者简介:
张鹏程(1990-),男,江苏启东人,博士研究生,从事重离子放射生物学基础研究;E-mail:pczhang@impcas.ac.cn

通讯作者: 李强,E-mail:liqiang@impcas.ac。

中图分类号: Q691

Radiosensitizing Effect of Gold Nanoparticles on Melanoma Cells Under Heavy Ion Irradiation

ZHANG Pengcheng^1,2,3,4,
LIU Yan^1,2,3,4,
JIN Xiaodong^1,2,3,4,
CHEN Weiqiang^1,2,3,4,
LI Qiang^{1,2,3,4
,}

1.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;
3.
Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou 730000, China;
4.
Nuclear Science and Technology School, University of Chinese Academy of Sciences, Beijing 100049, China

Funds: National Key Research and Development Program of China (2018YFC0115700, 2018YFC0115702);Major Project of Gansu Provincial Science and Technology (1602FKDA005);National Natural Science Foundation of China (11875299, 11805247)

摘要: 采用配体交换法合成了粒径15 nm左右的11-巯基十一烷酸包被的金纳米粒子（mAuNPs），使用透射电镜和纳米粒度电位仪对合成后的金纳米粒子进行了表征，然后用MTT法检测了mAuNPs对体外培养小鼠黑色素瘤B16-F10细胞的毒性。在传能线密度（LET）为50 keV/μm的碳离子束照射下，利用香豆素-3-羧酸（3CCA）作为荧光探针检测mAuNPs对水溶液中羟自由基的增强效应、二氯荧光素双醋酸盐（DCFHDA）检测mAuNPs对细胞内活性氧（ROS）的增强效应、克隆形成法检测mAuNPs对B16-F10细胞的辐射增敏效应。实验结果表明：mAuNPs对小鼠黑色素瘤B16-F10细胞基本无毒；mAuNPs对水溶液中的羟自由基产额增强为1.08~2.95倍；在共培养浓度为5 μg/mL情况下mAuNPs增加了胞内活性氧水平，mAuNPs在10%细胞存活水平下的辐射增敏比（SER）为1.15。因此，mAuNPs在黑色素瘤细胞中展现出对重离子的辐射增敏效应。
- 金纳米粒子(AuNPs) /
- 小鼠黑色素瘤细胞 /
- 辐射增敏效应 /
- 碳离子辐射
Abstract: Gold nanoparticles coated with 11-mercaptoundecanoic acid (mAuNPs) in diameter of 15 mm were synthesized using the ligand exchange method. The synthesized gold nanoparticles mAuNPs were characterized by transmission electron microscopy and Zetasizer. Then, the MTT assay was used to evaluate the toxicity of mAuNPs to mouse melanoma B16-f10 cells. All subsequent irradiation experiments were performed under a carbon ion beam with a linear energy transfer (LET) value of 50 keV/μm. First, coumarin-3-carboxylic acid (3-CCA) was used as a fluorescent probe to detect the radiation enhancement effect of mAuNPs on hydroxyl radicals in aqueous solution. Second, dichlorofluorescein diacetate (DCFH-DA) was used to evaluate the radiation enhancement effect of mAuNPs on intracellular reactive oxygen species (ROS). More importantly, the radiosensitizing effect of mAuNPs on B16-F10 cells under irradiation with carbon ions was assessed with the clonogenic survival assay. Our experimental results showed that mAuNPs had nearly no toxicity to mouse melanoma B16-F10 cells. The yield of hydroxide radicals in ultra-pure water in the presence of mAuNPs after exposure to carbon ions increased by a factor of 1.08~2.95. At a co-culture concentration of 5 μg/mL, mAuNPs increased the level of intracellular ROS and the sensitizer enhancement ratio (SER) of mAuNPs in B16-F10 cells at 10% survival level was 1.15. Thus, our study indicates that mAuNPs have radiation enhancement effect on melanoma cells under heavy ion irradiation.
- gold nanoparticles (AuNPs) /
- mouse melanoma cell /
- radiosensitizing effect /
- carbon ion radiation

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金纳米粒子对黑色素瘤细胞的重离子辐射增敏效应研究

doi: 10.11804/NuclPhysRev.36.04.492

1. 中国科学院近代物理研究所, 兰州 730000;

2. 中国科学院重离子束辐射生物医学重点实验室, 兰州 730000;

3. 甘肃省重离子束辐射医学应用基础重点实验室, 兰州 730000;

4. 中国科学院大学核科学与技术学院, 北京 100049

作者简介:
张鹏程(1990-),男,江苏启东人,博士研究生,从事重离子放射生物学基础研究;E-mail:pczhang@impcas.ac.cn

通讯作者: 李强,E-mail:liqiang@impcas.ac。

收稿日期: 2019-01-03
修回日期: 2019-05-07
刊出日期: 2019-12-20

中图分类号: Q691

关键词:

摘要: 采用配体交换法合成了粒径15 nm左右的11-巯基十一烷酸包被的金纳米粒子（mAuNPs），使用透射电镜和纳米粒度电位仪对合成后的金纳米粒子进行了表征，然后用MTT法检测了mAuNPs对体外培养小鼠黑色素瘤B16-F10细胞的毒性。在传能线密度（LET）为50 keV/μm的碳离子束照射下，利用香豆素-3-羧酸（3CCA）作为荧光探针检测mAuNPs对水溶液中羟自由基的增强效应、二氯荧光素双醋酸盐（DCFHDA）检测mAuNPs对细胞内活性氧（ROS）的增强效应、克隆形成法检测mAuNPs对B16-F10细胞的辐射增敏效应。实验结果表明：mAuNPs对小鼠黑色素瘤B16-F10细胞基本无毒；mAuNPs对水溶液中的羟自由基产额增强为1.08~2.95倍；在共培养浓度为5 μg/mL情况下mAuNPs增加了胞内活性氧水平，mAuNPs在10%细胞存活水平下的辐射增敏比（SER）为1.15。因此，mAuNPs在黑色素瘤细胞中展现出对重离子的辐射增敏效应。

English Abstract

Radiosensitizing Effect of Gold Nanoparticles on Melanoma Cells Under Heavy Ion Irradiation

1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

2. Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China;

3. Key Laboratory of Basic Research on Heavy Ion Radiation Application in Medicine, Gansu Province, Lanzhou 730000, China;

4. Nuclear Science and Technology School, University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2019-01-03
Rev Recd Date: 2019-05-07
Publish Date: 2019-12-20

Keywords:

Abstract: Gold nanoparticles coated with 11-mercaptoundecanoic acid (mAuNPs) in diameter of 15 mm were synthesized using the ligand exchange method. The synthesized gold nanoparticles mAuNPs were characterized by transmission electron microscopy and Zetasizer. Then, the MTT assay was used to evaluate the toxicity of mAuNPs to mouse melanoma B16-f10 cells. All subsequent irradiation experiments were performed under a carbon ion beam with a linear energy transfer (LET) value of 50 keV/μm. First, coumarin-3-carboxylic acid (3-CCA) was used as a fluorescent probe to detect the radiation enhancement effect of mAuNPs on hydroxyl radicals in aqueous solution. Second, dichlorofluorescein diacetate (DCFH-DA) was used to evaluate the radiation enhancement effect of mAuNPs on intracellular reactive oxygen species (ROS). More importantly, the radiosensitizing effect of mAuNPs on B16-F10 cells under irradiation with carbon ions was assessed with the clonogenic survival assay. Our experimental results showed that mAuNPs had nearly no toxicity to mouse melanoma B16-F10 cells. The yield of hydroxide radicals in ultra-pure water in the presence of mAuNPs after exposure to carbon ions increased by a factor of 1.08~2.95. At a co-culture concentration of 5 μg/mL, mAuNPs increased the level of intracellular ROS and the sensitizer enhancement ratio (SER) of mAuNPs in B16-F10 cells at 10% survival level was 1.15. Thus, our study indicates that mAuNPs have radiation enhancement effect on melanoma cells under heavy ion irradiation.

引用本文: