Research on Contemporary Damage Effects of Carbon Ion Beams with Different LET Irradiation on Dry Seeds of Arabidopsis thaliana
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摘要: 本研究以拟南芥(Columbia野生型)干种子为材料,利用兰州重离子研究装置(HIRFL)产生的碳离子束对材料进行辐射处理,统计其存活率、根长、下胚轴长及每果荚种子数,以探讨不同传能线密度(Linear Energy Transfer,LET)的碳离子束辐照对拟南芥当代损伤效应的影响。结果表明,在相同LET辐射条件下,随着辐射剂量的增大,拟南芥的存活率、根长、下胚轴长度、每果荚种子数都呈现下降趋势。在相同剂量不同LET辐射处理情况下,随着LET的增大,存活率、根长、下胚轴长、每果荚种子数都显著下降,可见高LET辐射严重抑制了拟南芥的生长和发育。研究表明,当LET为50 keV/μm时,碳离子束辐射拟南芥干种子对应的最佳诱变剂量为200 Gy,为后续开展碳离子束辐射的诱变效率研究奠定了前期基础。
Aimed to study the biological effects of carbon ion beams with different linear energy transfer (LET) values provided by Heavy Ion Research Facility in Lanzhou (HIRFL), dry seeds of Arabidopsis thaliana (Columbia-WT) were irradiated and a series of biological effects of postembryonic development, such as survival rate, primary root length, hypocotyls length and number of seeds per silique, were investigated. The results showed that, under the radiation condition of the same LET value, the survival rate, root length, hypocotyls length and number of seeds per silique were decreased with the increasing dose. In addition, under the radiation conditions with different LET values, but same dose, the extent of the decline of the survival rate, root length, hypocotyls length and number of seeds per silique were reinforced with the increasing LET. It was also found that high LET radiations inhibited the subsequent growth and development of Arabidopsis thaliana severely. In brief, it was suggested that the optimum dose of carbon ion beam with 50 keV/μm value on Arabidopsis thaliana dry seeds was 200 Gy. This research complemented the preliminary theoretical foundation for the comparative study of the highest mutation efficiency of carbon ion beam irradiations at IMP, CAS(Institute of Modern Physics, Chinese Academy of Sciences).Abstract: Aimed to study the biological effects of carbon ion beams with different linear energy transfer (LET) values provided by Heavy Ion Research Facility in Lanzhou (HIRFL), dry seeds of Arabidopsis thaliana (Columbia-WT) were irradiated and a series of biological effects of postembryonic development, such as survival rate, primary root length, hypocotyls length and number of seeds per silique, were investigated. The results showed that, under the radiation condition of the same LET value, the survival rate, root length, hypocotyls length and number of seeds per silique were decreased with the increasing dose. In addition, under the radiation conditions with different LET values, but same dose, the extent of the decline of the survival rate, root length, hypocotyls length and number of seeds per silique were reinforced with the increasing LET. It was also found that high LET radiations inhibited the subsequent growth and development of Arabidopsis thaliana severely. In brief, it was suggested that the optimum dose of carbon ion beam with 50 keV/μm value on Arabidopsis thaliana dry seeds was 200 Gy. This research complemented the preliminary theoretical foundation for the comparative study of the highest mutation efficiency of carbon ion beam irradiations at IMP, CAS(Institute of Modern Physics, Chinese Academy of Sciences).-
Key words:
- carbon ion beam /
- arabidopsis thaliana /
- linear energy transfer /
- irradiation
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[1] DU Yan. Mutation screening and mutagenic effects research of carbon-ion irradiation on Arabidopsis thaliana[D]. Graduate University of Chinese Academy of Sciences(Institute of Modern Physics), 2015. (in Chinese) (杜艳. 碳离子束辐照拟南芥突变体筛选及诱变效应研究[D]. 中国科学院研究生院(近代物理研究所), 2015.) [2] FENG Hui, LUO Shanwei, DU Yan, et al. Journal of Radiation Research and Radiation Processing, 2016, 34(1):10401.(in Chinese) (冯慧, 骆善伟, 杜艳, 等. 辐射研究与辐射工艺学报, 2016, 34(1):10401.) [3] WU Dali, HOU Suiwen, LIWenjian. Nuclear Physics Review, 2008, 25(3):287. (in Chinese) (吴大利, 侯岁稳, 李文建. 原子核物理评论, 2008, 25(3):287.) [4] QU Ying, LI Wenjin, ZHOU Libin, et al. Nuclear Physics Review, 2007, 24(4):294. (in Chinese) (曲颖, 李文建, 周利斌, 等. 原子核物理评论, 2007, 24(4):294.) [5] KIKUCHI S, SAITO Y, RYUTO H, et al. Mutation Research/fundamental & Molecular Mechanisms of Mutagenesis, 2009, 669(1-2):63. [6] NAOYA S, YUKIHIKO Y, SATOSHI K, et al. Genetics, 2003, 163(4):1449. [7] ROMANO F, CIRRONE G A P, CUTTONE G, et al. Physics in Medicine & Biology, 2014, 59(12):2863. [8] BELLI M, CERA F, CHERUBINI R, et al. International Journal of Radiation Biology, 1998, 74(4):501. [9] GERWECK L E, KOZIN S V. Radiotherapy & Oncology Journal of the European Society for Therapeutic Radiology & Oncology, 1999, 50(2):135. [10] SUZUKI M, KASE Y, KANAI T, et al. International Journal of Radiation Biology, 1997, 72(5):497. [11] MIZUHO A N, YOSHIYA F. J Radiat Res, 2013, 54(6):1029. [12] KAZAMA Y, HIRANO T, SAITO H, et al. Bmc Plant Biology, 2011, 11(1):284. [13] HIRANO T, KAZAMA Y, OHBU S, et al. Mutation Research/fundamental & Molecular Mechanisms of Mutagenesis, 2012, 735(1-2):19. [14] ZHOU Libin, LI Wenjian, QU Ying, et al. Journal of Radiation Research and Radiation Processing, 2007, 25(4):232(in Chinese) (周利斌, 李文建, 曲颖, 等. 辐射研究与辐射工艺学报, 2007, 25(4):232.) [15] ATSUSHI T, NAOYA S, YOSHIHIRO H. J Radiat Res, 2010, 51(3):223. [16] GREENE E A, CODOMO C A, TAYLOR N E, et al. Genetics, 2003, 164(2):731. [17] KRYSAN P J, YOUNG J C, SUSSMAN M R. Plant Cell, 1999, 11(12):2283. [18] ALONSO J M, STEPANOVA A N, LEISSE T J, et al. Science, 2003, 301(5633):653. [19] SHIKAZONO N, TANAKA A, KITAYAMA S, et al. Biophysik, 2002, 41(2):159. [20] QIN H L, XUE J M, LAI J N, et al. Nuclear Instruments & Methods in Physics Research, 2006, 245(1):314. [21] QIAGN Weiya, TANG Hongguan, HOU Zongdong, et al. Acta Ecologica Sinica, 2004, 24(4):852.(in Chinese) (强维亚, 汤红官, 侯宗东, 等. 生态学报, 2004, 24(4):852.) [22] WEI Zengquan, XIE Hongmei, LIANG Jianping, et al. Nuclear Physics Review, 2003, 20(1):38. (in Chinese) (卫增泉, 颉红梅, 梁剑平, 等. 原子核物理评论, 2003, 20(1):38.) -
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