Quantum Dots as a Probe to Observe the Process of Cell Damage Induced by Particle Irradiation
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摘要: 荧光量子点由于具有发射荧光效率高、光稳定性好、无光漂白等优良特性,因此在生物学研究中得到广泛应用。用量子点标记细胞,通过量子点荧光可以对细胞进行长时程观察,实现活细胞动态示踪。利用量子点和DiO 荧光染料对A549 细胞膜双染,直观展示了量子点优于有机荧光染料的抗光漂白特性,并阐述了一种利用量子点对细胞A549 进行标记并观测在 粒子辐照下细胞损伤动态变化过程的方法,这种方法对于阐明粒子辐射作用细胞的过程、了解辐射损伤的机理有一定的优势。Owing to the excellent optical properties such as high quantum yield of emission, photo-stability and anti-photobleaching, uorescent quantum dots(QDs) now have been extensively applied in bioscience research. Labeling cells by quantum dots allow long-term observation of the cellular processes. In this paper, we employed both QDs and DiO dye to label cells and thus showed the superior anti-photobleaching property of QDs directly, and also reported the method of application of QDs in observing the damage process of A549 cells induced by particle irradiation, demonstrating the advantage of application of QDs in the radiobiology research.Abstract: Owing to the excellent optical properties such as high quantum yield of emission, photo-stability and anti-photobleaching, uorescent quantum dots(QDs) now have been extensively applied in bioscience research. Labeling cells by quantum dots allow long-term observation of the cellular processes. In this paper, we employed both QDs and DiO dye to label cells and thus showed the superior anti-photobleaching property of QDs directly, and also reported the method of application of QDs in observing the damage process of A549 cells induced by particle irradiation, demonstrating the advantage of application of QDs in the radiobiology research.
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Key words:
- quantum dot /
- A549 cell /
- particle radiation
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