关键词: 
pH /
菌体浓度 /
糖含量 /
氮含量 /
代谢效应

摘要: 对¹²C⁶⁺ 离子辐照诱变高产阿维链霉菌株ZJAV-Y1-203 和原始菌株ZJAV-A-1 的摇瓶发酵pH 值、菌体浓度、碳源和氮源代谢进行了测定，研究了¹²C⁶⁺ 辐照对阿维链霉菌的代谢效应。在发酵前期(48h)，原始菌株发酵液pH 值低于突变菌株；在发酵96~196 h，诱变高产菌株ZJAV-Y1-203 繁殖快，生长旺盛，N的利用率高；菌体浓度大于原始菌株的浓度，且发酵液的pH 稳定，菌体处于代谢相对更稳定期；在发酵144~240 h，诱变高产菌株ZJAV-Y1-203 对糖源消耗低于原始菌株ZJAV-A-1。这些结果表明，¹²C⁶⁺ 离子辐照对阿维链霉菌代谢影响有利于阿维菌素合成。pH value, mycelium concentration, carbon source and nitrogen metabolism in flask fermentation of the mutant high-producing strain ZJAV-Y1-203 and the original strain ZJAV-A1 have been investigated, in order to show the metabolic effect of avermitilis irradiated by ion beam of ¹²C⁶. In early stage (48 h) of the fermentation, pH value of the original fermentation was lower than that of the mutant strains. In 96~196 h of fermentation, the nitrogen utilization in the strains ZJAV-Y1-203 was higher than that in the original strains, its reproductive was fast, and its growing was vigorous. The mycelium concentration of ZJAV-Y1-203 was greater than the original strain, and the pH value of fermentation were stable, so its metabolism was relatively more stable. In 144240 h of fermentation, the strain ZJAV-Y1-203 on sugar consumption was less than the original strains. The effect of ¹²C⁶ ion irradiation on metabolism of Streptomyces avermitilis is conducive to the synthesis of avermectin.