关键词: 
离子注入 /
UV-B /
POD /
SOD /
MDA /
GSH

摘要:  采用3种不同注量的N离子对供试材料进行了离子注入预处理， 再用10.08 kJ/(m²d¹)增强UV-B辐射对其幼苗进行辐照试验， 测定了过氧化物酶（POD）活性、 过氧化物歧化酶（SOD）活性、 过氧化氢酶（CAT）活性、 丙二醛（MDA）含量、 还原型谷胱甘肽（GSH）含量和可溶性糖含量的变化。 研究结果表明： 经N离子注入预处理的水稻材料受到UV-B辐射时其POD和SOD活性明显升高， 并在注量为2.0×10¹⁷ ions/cm²时活性达到最强； CAT活性和MDA含量明显降低， GSH含量显著增加， 而可溶性糖的含量则变化程度很小。 从这些研究结果可以看出， 经过一定注量离子注入预处理的水稻材料当受到UVB辐射时能产生较强的抗氧化能力和防御能力，而且抗氧化系统优于碳水化合物系统而先于表达。 由此可见， 离子注入预处理对水稻UV-B辐射的生物学效应的影响相当明显。 The seedlings of rice (xindao18) were exposed to UVB (10.08 kJ/(m²d¹ )) irradiation following the pretreatment with three different implantation dosages of lowenergy N⁺ ions. Changes in the levels of the superoxide (POD)，superoxide dismutase (SOD)，catalase (CAT)， malondialdehyde (MDA)，glutathione (GSH) and soluble sugar were measured. The result showed that the UV-B irradiation on the seedlings of rice pretreated with lowenergy ions implantation could lead to increase activities in POD and SOD，and the maximum appeared on the dose of 2.0×10¹⁷ ions/cm². Meanwhile，it made the content of GSH increased， and caused the activity of CAT and the content of MDA to be decreased. But there was no obvious change in soluble sugar. It was suggested that the rice pretreated by low energy ion implantation could enhance the antioxidation capacity and defensive ability when irradiated by UV-B， and the antioxidation system could be induced earlier than carbohydrate system. Therefore， the biological effects of UV-B irradiation on rice pretreated by low energy ion implantation were quite obvious.