关键词: 
¹²C⁶⁺离子束 /
辐照剂量 /
皮肤 /
猪 /
组织病理学

摘要: 探讨了正常皮肤对重离子辐照急性损伤反应的耐受性， 为重离子治癌临床应用提供安全性检测的实验依据。实验前10 min， 实验猪肌肉注射复方氯胺酮1.2 mg/kg进行麻醉， 然后在兰州重离子研究装置（HIRFL）辐照终端利用12C6+束照射， 辐照剂量分别为0, 12， 21和27 Gy， 辐照分3次完成， 剂量率约为1.2 Gy/min， Bragg峰区照射， 辐照后每隔7 d对照射野拍照并活检取样, 做HE组织病理学观察。不同剂量¹²C⁶⁺离子束辐照实验猪皮肤后, 皮肤外观反应随辐照剂量增大而加快， 表现为肿胀和色素沉积等； 皮肤组织结构的变化明显， 上皮细胞排列紊乱、 萎缩、 空泡变性； 基本恢复正常所需时间也越长， 且都存在明显的剂量效应关系。结果表明, 辐照剂量范围为0—27 Gy时, 重离子对正常皮肤的辐照是安全的。The tolerance of the normal skin to the acute radiation injury reaction induced by heavy ion beams has been studied experimentally. The experimental pigs were injected with 1.2 mg/kg ketamine in 10 min before irradiation and were irradiated with 0， 12 ， 21 and 27 Gy 12C6+ ion at a dose rate of 1.2 Gy/min at the Heavy Ion Research Facility in Lanzhou（HIRFL）. The total radiation dose was finished by 3 times at Bragg Peak Region of Heavy Ion Beams.The radiation fields of skin were taken photo and performed biopsy. The contaneous tissues of radiation fields were stained by HE and examined histopatholo gical changes every seven days after irradiation. The results indicated that the cutaneous appearance reaction became more faster with radiation dosage rising and presented with swollen， melanin forming and so on after irradiated by the carbon ions at different dosage. The Pathological examination showed noticeable changes in histological and structural of experimental pigs skin， such as atrophy， vacuolation， denaturation and arranged irregularly in epithelial cells. Furthermore， the time for return to normality became longer with the increasing of radiation dosage. All indexes demonstrated correlation between the does and effects. It is concluded that the irradiation of heavy ion beams to normal skin is security when the radiation dose range is about 0—27 Gy.