低能N<SUP>+</SUP>注入E.<EM>coli</EM> 诱发的HRS/IRR与抗氧化物酶关系的初探

杨天佑; 王铁固; 田静; 常景玲; 秦广雍

doi:10.11804/NuclPhysRev.27.02.197

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低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

杨天佑, 王铁固, 田静, 常景玲, 秦广雍

文章导航 > 原子核物理评论 > 2010 > 27(2): 197-201

杨天佑, 王铁固, 田静, 常景玲, 秦广雍. 低能N+注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探[J]. 原子核物理评论, 2010, 27(2): 197-201. doi: 10.11804/NuclPhysRev.27.02.197

引用本文:

杨天佑, 王铁固, 田静, 常景玲, 秦广雍. 低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探[J]. 原子核物理评论, 2010, 27(2): 197-201. doi: 10.11804/NuclPhysRev.27.02.197

YANG Tian-you, WANG Tie-gu, TIAN Jing, CHANG Jing-ling. Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N+[J]. Nuclear Physics Review, 2010, 27(2): 197-201. doi: 10.11804/NuclPhysRev.27.02.197

Citation:

YANG Tian-you, WANG Tie-gu, TIAN Jing, CHANG Jing-ling. Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N⁺[J]. Nuclear Physics Review, 2010, 27(2): 197-201. doi: 10.11804/NuclPhysRev.27.02.197

低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

doi: 10.11804/NuclPhysRev.27.02.197

1 河南科技学院生命科技学院, 河南新乡 453003; 2 郑州大学离子束生物工程省重点实验室, 河南郑州 450052

Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N⁺

1 Life Science and Technology College, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China;2 Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China

摘要: 研究了30 keV N+注入E.coli 诱发的辐射超敏感效应/增强的辐射性(HRS/IRR)及该效应诱发时E.coli细胞内抗氧化物酶(SOD和CAT)的诱导表达，探讨了细胞内抗氧化物酶的诱导与HRS/IRR诱发之间的关联。研究结果显示， HRS诱发剂量范围内（0—5×1014 N+/cm2）， E.coli细胞内超氧化物岐化酶(SOD)的酶活基本维持在10 Us/mg的较低水平，过氧化氢敏(CAT)酶活也都低于8 Us/mg； IRR诱发的剂量范围内（5×1014—10×1014 N+/cm2 ）， SOD和CAT活性升高并分别达到了58 Us/mg和26 Uc/mg的最高水平。研究结果初步表明，低能离子注入E.coli HRS/IRR的诱发与细胞内抗氧化物酶的诱导关系密切。 The inducement of hyperradiosensitivity/increased radioresistance(HRS/IRR) and activities of antioxidases in Escherichia coli implanted by 30 keV N⁺ and relationship between them were investigated. The results showed the change trend of the survival rate and the activity of superoxide dismutase(SOD) and Catalas(CAT) of E. coli K12 was consistent. The activity of SOD and CAT of E.coli were lower， less than 10 and 8 U/mg， when the HRS was induced at 0—5×10¹⁴  N⁺/cm² doses. And the activity of SOD and CAT of E.coli increased and reached highest rate， were 58 and 26 U/mg respectively， when the IRR was induced at 5×10¹⁴—10×10¹⁴ N⁺/cm² doses. It could be concluded that the change of SOD and CAT activity was related with the HRS/IRR.
- N+注入 /
- 辐射超敏感效应 /
- 增强的辐射性 /
- 超氧化物岐化酶 /
- 过氧化氢酶
Abstract: The inducement of hyperradiosensitivity/increased radioresistance(HRS/IRR) and activities of antioxidases in Escherichia coli implanted by 30 keV N⁺ and relationship between them were investigated. The results showed the change trend of the survival rate and the activity of superoxide dismutase(SOD) and Catalas(CAT) of E. coli K12 was consistent. The activity of SOD and CAT of E.coli were lower， less than 10 and 8 U/mg， when the HRS was induced at 0—5×10¹⁴  N⁺/cm² doses. And the activity of SOD and CAT of E.coli increased and reached highest rate， were 58 and 26 U/mg respectively， when the IRR was induced at 5×10¹⁴—10×10¹⁴ N⁺/cm² doses. It could be concluded that the change of SOD and CAT activity was related with the HRS/IRR.
- N⁺implantion /
- hyperradiosensitivity /
- increased radioresistance /
- superoxide dismutase /
- catalas

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低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

doi: 10.11804/NuclPhysRev.27.02.197

1 河南科技学院生命科技学院, 河南新乡 453003; 2 郑州大学离子束生物工程省重点实验室, 河南郑州 450052

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2010-06-20

关键词:

N+注入 /

摘要: 研究了30 keV N+注入E.coli 诱发的辐射超敏感效应/增强的辐射性(HRS/IRR)及该效应诱发时E.coli细胞内抗氧化物酶(SOD和CAT)的诱导表达，探讨了细胞内抗氧化物酶的诱导与HRS/IRR诱发之间的关联。研究结果显示， HRS诱发剂量范围内（0—5×1014 N+/cm2）， E.coli细胞内超氧化物岐化酶(SOD)的酶活基本维持在10 Us/mg的较低水平，过氧化氢敏(CAT)酶活也都低于8 Us/mg； IRR诱发的剂量范围内（5×1014—10×1014 N+/cm2 ）， SOD和CAT活性升高并分别达到了58 Us/mg和26 Uc/mg的最高水平。研究结果初步表明，低能离子注入E.coli HRS/IRR的诱发与细胞内抗氧化物酶的诱导关系密切。 The inducement of hyperradiosensitivity/increased radioresistance(HRS/IRR) and activities of antioxidases in Escherichia coli implanted by 30 keV N⁺ and relationship between them were investigated. The results showed the change trend of the survival rate and the activity of superoxide dismutase(SOD) and Catalas(CAT) of E. coli K12 was consistent. The activity of SOD and CAT of E.coli were lower， less than 10 and 8 U/mg， when the HRS was induced at 0—5×10¹⁴  N⁺/cm² doses. And the activity of SOD and CAT of E.coli increased and reached highest rate， were 58 and 26 U/mg respectively， when the IRR was induced at 5×10¹⁴—10×10¹⁴ N⁺/cm² doses. It could be concluded that the change of SOD and CAT activity was related with the HRS/IRR.

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低能N+注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

doi: 10.11804/NuclPhysRev.27.02.197

Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N+

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低能N+注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

doi: 10.11804/NuclPhysRev.27.02.197

1 河南科技学院生命科技学院, 河南 新乡 453003; 2 郑州大学离子束生物工程省重点实验室, 河南 郑州 450052

English Abstract

Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N+

1 Life Science and Technology College, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China;2 Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China

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低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N⁺

低能N⁺注入E.coli 诱发的HRS/IRR与抗氧化物酶关系的初探

1 河南科技学院生命科技学院, 河南新乡 453003; 2 郑州大学离子束生物工程省重点实验室, 河南郑州 450052

Research on Relation of HRS/IRR and Antioxidases in E.coli Implanted by 30 keV N⁺