关键词: 
辐照诱变 /
阿维菌素B1 /
效价 /
高产菌株

摘要: 选用¹²C⁶⁺离子辐照诱变阿维菌素B1a产生菌ZJAV-A1， 研究其诱变效应。 实验结果表明， ¹²C⁶⁺离子辐照剂量50 Gy时致死率97%，正突变率最高可达到34.2%。 通过¹²C⁶⁺离子诱变处理，结合平板培养基及斜面培养基的正突变菌株筛选， 最终获得一株稳定性良好， 阿维菌素B1a组分产量稳定在4460—4588 μg/ml之间， 较出发菌株提高11.1%—14.7%的突变株ZJAVY1203。 Mutagenic effect on avermectin B1a producing strains of ZJAV-A1 by ion beam of ¹²C⁶⁺ has been investigated.The experimental results indicated that the lethality was 97% and the highest rate of orthomutation was 34.2%， when ZJAVA1 was irradiated by ion beam of 50 Gy ¹²C⁶⁺. After the mutagenesis processing by ion beam of ¹²C⁶⁺ and the screening of orthomutation strains by using plating mediums and slant cultures，the mutant ZJAV-Y1203 was obtained with theavermectin B1a yield of 4588—4460 μg/ml. Compared with the original strain， the titer was improved 11.1%—14.7%.