关键词: 
黑松 /
花粉粒 /
细胞核 /
离子注入 /
损伤效应

摘要: 利用激光扫描共聚焦显微技术和碱性单细胞凝胶电泳技术对经过离子注入后的黑松花粉粒内细胞核的直接损伤效应进行了观察鉴定。 研究结果表明， 离子注入后可以直接损伤细胞核结构， 导致细胞核裂解。 细胞核的损伤程度与注入离子的剂量密切相关， 即细胞核DNA分子的损伤程度随着注入离子剂量的增加而提高。 The damage of pollen nuclei of Pinus thunbergii induced directly by ion implantation were measured by the laser confocal microscopy technique and the singlecell gel electrophoresis test. The results showed that ion implantation caused the nuclei structure to be damaged， leading to the nuclei degradation. The results of statistical analysis showed that the damage grade of nuclei was very correlative with the ion dosage. The damage degree of DNA in the nuclei at the level of single cells was increased with the increase of the ion implantation dose.