X射线辐照对发根农杆菌介导芸芥遗传转化的影响

武振华; 张红; #; 牛炳韬; 李莎; 王根轩; 孙鹏; 刘斌; 李宁

doi:10.11804/NuclPhysRev.25.04.414

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X射线辐照对发根农杆菌介导芸芥遗传转化的影响

武振华, 张红, #, 牛炳韬, 李莎, 王根轩, 孙鹏, 刘斌, 李宁

文章导航 > 原子核物理评论 > 2008 > 25(4): 414-418

武振华, 张红, #, 牛炳韬, 李莎, 王根轩, 孙鹏, 刘斌, 李宁. X射线辐照对发根农杆菌介导芸芥遗传转化的影响[J]. 原子核物理评论, 2008, 25(4): 414-418. doi: 10.11804/NuclPhysRev.25.04.414

引用本文:

WU Zhen-hua, ZHANG Hong, #, NIU Bing-tao, LI Sha, WANG Gen-xuan, SUN Peng, LIU Bin, LI Ning. Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes[J]. Nuclear Physics Review, 2008, 25(4): 414-418. doi: 10.11804/NuclPhysRev.25.04.414

Citation:

WU Zhen-hua, ZHANG Hong, #, NIU Bing-tao, LI Sha, WANG Gen-xuan, SUN Peng, LIU Bin, LI Ning. Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes[J]. Nuclear Physics Review, 2008, 25(4): 414-418. doi: 10.11804/NuclPhysRev.25.04.414

X射线辐照对发根农杆菌介导芸芥遗传转化的影响

doi: 10.11804/NuclPhysRev.25.04.414

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 兰州大学生命科学学院, 甘肃兰州 730000; 3 兰州军区陆军总医院, 甘肃兰州 730000; 4 甘肃省建筑工程总公司第一中学, 甘肃兰州 730000

详细信息

通讯作者: 张红

Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 School of Life Science, Lanzhou University, Lanzhou 730000, China; 3 General Hospital of Lanzhou Military Area, Lanzhou 730050, China; 4 First Middle School, Gansu Construction Engineering Corporation, Lanzhou 730000, China

摘要: 探讨了X射线辐照对芸芥发状根诱导的影响，为研究辐射对转基因技术的协同作用提供一定的实验依据。以芸芥无菌苗的子叶为材料，通过5—20 Gy的X射线辐照和不等浸染时间的联合处理，研究苗龄、预培养时间、辐照剂量及其浸染时间等因素对芸芥发状根诱导的影响作用，并通过聚合链酶式反应（PCR）对所得发状根进行了分子水平的鉴定。 15 Gy X射线辐射能提高芸芥发状根的诱导频率，且有量效关系，其中先浸染后辐照比先辐照后浸染效果更显著； PCR结果也表明，发根农杆菌R1000的 rolB基因已成功地被转入并整合到该发状根的基因组中。 X射线辐照对芸芥发状根的诱导具有一定的促进作用，且最佳推荐诱导剂量为15 Gy或稍多。
- X射线 /
- 辐照 /
- 芸芥 /
- 发状根
Abstract: To investigate the effects of X-ray irradiation to the hairy root induction of Eruca sativa Mill， and to provide basic experimental data for concerted reaction of irradiation to the Genetic transformation technology. The aseptic cotyledons of Eruca sativa Mill were jointly treated with the Xray at dose of 5， 10， 15， 20 Gy respectively and the different infected time， then the influence of explant age， preculturing time， dose of Xray and infected time were studied. Furthermore， the hairy roots were testified in molecular level by the polymerase chain reaction（PCR）. The irradiation of Xray at dose of 15 Gy can improve the frequency of Eruca sativa Mill hairy root induction in a dosedependent manner. Moreover， the transformation frequency in preinfection plus irradiation group is higher than that in preirradiation plus infection group at the same irradiation dose. In addition， the PCR analysis also demonstrated that rolB gene of TDNA from Ri plasmid had been integrated into the genome of the transformed roots. The irradiation of Xray has some positive effects on the hairy root induction of Eruca sativa Mill， and the optimal revulsive dose is 15 Gy or a little bit more.
- X-ray /
- radiation /
- Eruca sativa Mill /
- hairy root

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X射线辐照对发根农杆菌介导芸芥遗传转化的影响

doi: 10.11804/NuclPhysRev.25.04.414

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 兰州大学生命科学学院, 甘肃兰州 730000; 3 兰州军区陆军总医院, 甘肃兰州 730000; 4 甘肃省建筑工程总公司第一中学, 甘肃兰州 730000

通讯作者: 张红

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2008-12-20

关键词:

X射线 /

辐照 /

芸芥 /

发状根

摘要: 探讨了X射线辐照对芸芥发状根诱导的影响，为研究辐射对转基因技术的协同作用提供一定的实验依据。以芸芥无菌苗的子叶为材料，通过5—20 Gy的X射线辐照和不等浸染时间的联合处理，研究苗龄、预培养时间、辐照剂量及其浸染时间等因素对芸芥发状根诱导的影响作用，并通过聚合链酶式反应（PCR）对所得发状根进行了分子水平的鉴定。 15 Gy X射线辐射能提高芸芥发状根的诱导频率，且有量效关系，其中先浸染后辐照比先辐照后浸染效果更显著； PCR结果也表明，发根农杆菌R1000的 rolB基因已成功地被转入并整合到该发状根的基因组中。 X射线辐照对芸芥发状根的诱导具有一定的促进作用，且最佳推荐诱导剂量为15 Gy或稍多。

English Abstract

Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 School of Life Science, Lanzhou University, Lanzhou 730000, China; 3 General Hospital of Lanzhou Military Area, Lanzhou 730050, China; 4 First Middle School, Gansu Construction Engineering Corporation, Lanzhou 730000, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Keywords:

Abstract: To investigate the effects of X-ray irradiation to the hairy root induction of Eruca sativa Mill， and to provide basic experimental data for concerted reaction of irradiation to the Genetic transformation technology. The aseptic cotyledons of Eruca sativa Mill were jointly treated with the Xray at dose of 5， 10， 15， 20 Gy respectively and the different infected time， then the influence of explant age， preculturing time， dose of Xray and infected time were studied. Furthermore， the hairy roots were testified in molecular level by the polymerase chain reaction（PCR）. The irradiation of Xray at dose of 15 Gy can improve the frequency of Eruca sativa Mill hairy root induction in a dosedependent manner. Moreover， the transformation frequency in preinfection plus irradiation group is higher than that in preirradiation plus infection group at the same irradiation dose. In addition， the PCR analysis also demonstrated that rolB gene of TDNA from Ri plasmid had been integrated into the genome of the transformed roots. The irradiation of Xray has some positive effects on the hairy root induction of Eruca sativa Mill， and the optimal revulsive dose is 15 Gy or a little bit more.

引用本文:

Citation:

WU Zhen-hua, ZHANG Hong, #, NIU Bing-tao, LI Sha, WANG Gen-xuan, SUN Peng, LIU Bin, LI Ning. Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes[J]. Nuclear Physics Review, 2008, 25(4): 414-418. doi: 10.11804/NuclPhysRev.25.04.414

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X射线辐照对发根农杆菌介导芸芥遗传转化的影响

doi: 10.11804/NuclPhysRev.25.04.414

通讯作者: 张红

Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes

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X射线辐照对发根农杆菌介导芸芥遗传转化的影响

doi: 10.11804/NuclPhysRev.25.04.414

1 中国科学院近代物理研究所, 甘肃 兰州 730000; 2 兰州大学生命科学学院, 甘肃 兰州 730000; 3 兰州军区陆军总医院, 甘肃 兰州 730000; 4 甘肃省建筑工程总公司第一中学, 甘肃 兰州 730000

通讯作者: 张红

English Abstract

Effects of Xray Irradiation to Genetic Transformation of Eruca sativa Mill Mediated by Agrobacterium rhizogenes

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1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 兰州大学生命科学学院, 甘肃兰州 730000; 3 兰州军区陆军总医院, 甘肃兰州 730000; 4 甘肃省建筑工程总公司第一中学, 甘肃兰州 730000