X射线致HeLa细胞损伤过程中NADPH氧化酶的作用

杜斌斌; 刘箐; 张维胜; 王晓妍; 贾鹏飞; 王小虎; 李莎; 杨熊飞; 张红; 郭天康; #

doi:10.11804/NuclPhysRev.25.04.391

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X射线致HeLa细胞损伤过程中NADPH氧化酶的作用

杜斌斌, 刘箐, 张维胜, 王晓妍, 贾鹏飞, 王小虎, 李莎, 杨熊飞, 张红, 郭天康, #

文章导航 > 原子核物理评论 > 2008 > 25(4): 391-396

杜斌斌, 刘箐, 张维胜, 王晓妍, 贾鹏飞, 王小虎, 李莎, 杨熊飞, 张红, 郭天康, #. X射线致HeLa细胞损伤过程中NADPH氧化酶的作用[J]. 原子核物理评论, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391

引用本文:

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391

Citation:

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391

X射线致HeLa细胞损伤过程中NADPH氧化酶的作用

doi: 10.11804/NuclPhysRev.25.04.391

1 兰州大学临床医学院, 甘肃兰州 730000; 2 中国科学院近代物理研究所, 甘肃兰州 730000; 3 甘肃省人民医院, 甘肃兰州 730000; 4 兰州大学生命科学学院, 甘肃兰州 730000; 5 甘肃省肿瘤医院, 甘肃兰州 730050; 6 兰州军区总医院, 甘肃兰州 730050

详细信息

通讯作者: 郭天康

Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation

1 Clinical Medical College, Lanzhou University, Lanzhou 730000, China;2 Institute Of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3 People’s Hospital of Gansu Province, Lanzhou 730000, China;4 School of Life Science, Lanzhou University, Lanzhou 730000, China;5 Gansu Tumor Hospital, Lanzhou 730050, China;6 General Hospital of Lanzhou Military Area, Lanzhou 730050, China

摘要: 以HeLa细胞为实验材料，探讨了NADPH氧化酶在X射线诱导细胞损伤过程中的作用。结果显示， 12 Gy X射线辐照后细胞内活性氧(ROS)明显增加，在用NADPH氧化酶抑制剂处理后再辐照，则细胞内ROS降低到未辐照水平；同时辐照后NADPH 氧化酶细胞质亚基p47^phox 在细胞质积聚并和细胞膜亚基 gp91phox 结合； Western blotting检测结果显示， NADPH 氧化酶的关键亚基 gp91phox 的表达量明显增加。以上结果说明， NADPH氧化酶可以被X射线激活，由其介导产生的ROS在X射线诱导HeLa细胞损伤过程中扮演重要角色。 To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.
- NADPH氧化酶 /
- 活性氧 /
- X射线 /
- HeLa细胞
Abstract: To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.
- NADPH oxidase /
- reactive oxygen species /
- Xray /
- HeLa cell

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X射线致HeLa细胞损伤过程中NADPH氧化酶的作用

doi: 10.11804/NuclPhysRev.25.04.391

1 兰州大学临床医学院, 甘肃兰州 730000; 2 中国科学院近代物理研究所, 甘肃兰州 730000; 3 甘肃省人民医院, 甘肃兰州 730000; 4 兰州大学生命科学学院, 甘肃兰州 730000; 5 甘肃省肿瘤医院, 甘肃兰州 730050; 6 兰州军区总医院, 甘肃兰州 730050

通讯作者: 郭天康

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2008-12-20

关键词:

NADPH氧化酶 /

活性氧 /

X射线 /

HeLa细胞

摘要: 以HeLa细胞为实验材料，探讨了NADPH氧化酶在X射线诱导细胞损伤过程中的作用。结果显示， 12 Gy X射线辐照后细胞内活性氧(ROS)明显增加，在用NADPH氧化酶抑制剂处理后再辐照，则细胞内ROS降低到未辐照水平；同时辐照后NADPH 氧化酶细胞质亚基p47^phox 在细胞质积聚并和细胞膜亚基 gp91phox 结合； Western blotting检测结果显示， NADPH 氧化酶的关键亚基 gp91phox 的表达量明显增加。以上结果说明， NADPH氧化酶可以被X射线激活，由其介导产生的ROS在X射线诱导HeLa细胞损伤过程中扮演重要角色。 To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.

English Abstract

Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation

1 Clinical Medical College, Lanzhou University, Lanzhou 730000, China;2 Institute Of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;3 People’s Hospital of Gansu Province, Lanzhou 730000, China;4 School of Life Science, Lanzhou University, Lanzhou 730000, China;5 Gansu Tumor Hospital, Lanzhou 730050, China;6 General Hospital of Lanzhou Military Area, Lanzhou 730050, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2008-12-20

Keywords:

Abstract: To investigate the role of NADPH oxidase in HeLa cell lesion induced by Xray irradiation, the change of cell survival was detected with MTT assay， reactive oxygen species (ROS) was measured by fluorospectrophotometer. Immunostaining and confocal laserscanning microscopy was employed to detect the colocalization of two subunit of NADPH oxidase, p47^phox and gp91^phox in the cell. Western blotting was used to detect the expression of gp91^phox before and after Xray irradiation. After Xray irradiation， intracellular level of ROS increased obviously. But the increase could be blocked by diphenyleneiodonium (DPI)， an inhibitor of NADPH oxidase. Meanwhile， cytosolic subunit p47^phox moved to membrane and colocalizated with gp91^phox after
irradiation. Moreover, the results also show that gp91^phox increased sharply after 12 Gy Xray irradiation. Therefore， NADPH oxidasemediated production of ROS plays an important role in HeLa cell lesion induced by Xray.

引用本文:

Citation:

DU Bin-bin, LIU Qing, ZHANG Wei-sheng, WANG Xiao-yan, JIA Pen-fei, WANG Xiao-hu, LI Sha, YANG Xiong-fei, ZHANG Hong, Guo Tian-kang, #. Role of NADPH Oxidase in HeLa Cell Lesion Induced by Xray Irradiation[J]. Nuclear Physics Review, 2008, 25(4): 391-396. doi: 10.11804/NuclPhysRev.25.04.391