High efficiency Transfer and Expression of AdCMV-p53 in Human Prostate Adenocarcinoma Cells Induced by Low dose 60Co γ ray
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摘要: 用腺病毒重组体(AdCMVp53/GFP)转染经0.5, 1.0和2.0 Gy γ射线辐射处理的前列腺癌细胞[PC3( nullp53)], 用克隆形成法检测细胞增殖能力, 用流式细胞分析法测定腺病毒重组体转染率和外源性p53蛋白表达。 结果提示, 辐射诱导使腺病毒重组体转染PC3细胞提高7%—39%。 辐射联合 AdCMVp53 转染组p53表达水平提高18.5%—35.4%。 与单纯 AdCMVp53 转染组和单纯辐射组相比, 辐射联合 AdCMV-p53 转染组细胞存活率分别降低25%—64%和22%—65%。 To determine whether lowdose preirradiation could enhance adenovirusmediated p53 transfer and expression in human prostate adenocarcinoma, the PC3 cells were preexposed to γ rays, and then infected with replicationdeficient adenovirus recombinant vectors, containing human wildtype p53 (AdCMVp53) or green fluorescent protein gene (AdCMVGFP) respectively (γray irradiation + AdCMVp53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMVGFP infection groups were 7%—39% more than those in AdCMVGFP infection groups. The p53 levels in the γ irradiation + AdCMVp53 infection groups were 18.5%—35.4% more than those in AdCMVp53 infection groups (p<0.05),suggesting that lowdose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC3 cells. The survival fractions for the γ irradiation + AdCMVp53 infection groups were 25%—64%, 22%—65% less than those for AdCMVp53 infection, or γirradiation groups, respectively (p<0.05).Abstract: To determine whether lowdose preirradiation could enhance adenovirusmediated p53 transfer and expression in human prostate adenocarcinoma, the PC3 cells were preexposed to γ rays, and then infected with replicationdeficient adenovirus recombinant vectors, containing human wildtype p53 (AdCMVp53) or green fluorescent protein gene (AdCMVGFP) respectively (γray irradiation + AdCMVp53 /GFP infection). The exogenous gene transfer and expression were detected by flow cytometric analysis. The GFP transfer frequencies in γ irradiation + AdCMVGFP infection groups were 7%—39% more than those in AdCMVGFP infection groups. The p53 levels in the γ irradiation + AdCMVp53 infection groups were 18.5%—35.4% more than those in AdCMVp53 infection groups (p<0.05),suggesting that lowdose (less than or equal to 1.0 Gy) irradiation could significantly promote exogenous p53 transfer and expression in the PC3 cells. The survival fractions for the γ irradiation + AdCMVp53 infection groups were 25%—64%, 22%—65% less than those for AdCMVp53 infection, or γirradiation groups, respectively (p<0.05).
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Key words:
- p53-gene /
- irradiation /
- adenovirus vector /
- prostate adenocarcinoma /
- radiosensitivity
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