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γ射线诱导DNA损伤中DNA浓度和剂量率的影响

孔福全,  王潇,  倪嵋楠,  隋丽,  杨明建,  赵葵,  #

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文章导航 > 原子核物理评论  > 2007 >  24(2): 103-107
孔福全, 王潇, 倪嵋楠, 隋丽, 杨明建, 赵葵, #. γ射线诱导DNA损伤中DNA浓度和剂量率的影响[J]. 原子核物理评论, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
引用本文: 孔福全, 王潇, 倪嵋楠, 隋丽, 杨明建, 赵葵, #. γ射线诱导DNA损伤中DNA浓度和剂量率的影响[J]. 原子核物理评论, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
shu
KONG Fu-quan, WANG Xiao, NI Mei-nan, SUI Li, YANG Ming-jian, . Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray[J]. Nuclear Physics Review, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
Citation: KONG Fu-quan, WANG Xiao, NI Mei-nan, SUI Li, YANG Ming-jian, . Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray[J]. Nuclear Physics Review, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
shu

γ射线诱导DNA损伤中DNA浓度和剂量率的影响

doi: 10.11804/NuclPhysRev.24.02.103

  • 孔福全1, 王潇1, 倪嵋楠1, 隋丽1, 杨明建1, 2, 赵葵1, 2, 3, #(1 中国原子能科学研究院, 北京102413; 2 河北工业大学理学院, 天津 300130;3 北京师范大学射线束技术与材料改性教育部重点实验室, 北京 100875)

详细信息
    通讯作者: 赵葵

Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray

  • 1 China Institute of Atomic Energy, Beijing 102413, China; 2 School of Science, Hebei University of Technology, Tianjin 300130, China;3 Key Laboratory of Beam Technology and Modification of Ministry of Education, Beijing Normal University, Beijing 100875, China

  • 摘要: 在进行辐射致DNA损伤中, DNA浓度和剂量率是两个重要的因素。 利用γ射线在不同的剂量率下对不同浓度的质粒DNA pUC19水溶液进行了辐照， 利用凝胶电泳对辐照后的样品进行分析。 结果表明， 在同一剂量下被辐照的DNA样品， 随浓度的降低DNA损伤越来越严重； 通过分析软件和理论公式得到的每个DNA上的双链断裂数随着DNA浓度的变化呈现为非线性趋势。 As two factors， the changes of concentration of DNA and dose rate are of significance in the study of DNA damage induced by irradiation. The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated. The result of gel electrophoresis indicate that DNA molecules were damaged severely as the concentration of DNA decreasing. The number of double strand breakages per DNA were obtained by the software of Alpha Innotech and theoretical analysis. It shows that the numbers of double strand breakages per DNA presented nonlinear feature as the concentration of DNA decreasing, and the dose rate had little influence on DNA damage.

    Abstract: As two factors， the changes of concentration of DNA and dose rate are of significance in the study of DNA damage induced by irradiation. The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated. The result of gel electrophoresis indicate that DNA molecules were damaged severely as the concentration of DNA decreasing. The number of double strand breakages per DNA were obtained by the software of Alpha Innotech and theoretical analysis. It shows that the numbers of double strand breakages per DNA presented nonlinear feature as the concentration of DNA decreasing, and the dose rate had little influence on DNA damage.
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出版历程
  • 收稿日期:  1900-01-01
  • 修回日期:  1900-01-01
  • 刊出日期:  2007-06-20

γ射线诱导DNA损伤中DNA浓度和剂量率的影响

doi: 10.11804/NuclPhysRev.24.02.103
  • 孔福全1, 王潇1, 倪嵋楠1, 隋丽1, 杨明建1, 2, 赵葵1, 2, 3, #(1 中国原子能科学研究院, 北京102413; 2 河北工业大学理学院, 天津 300130;3 北京师范大学射线束技术与材料改性教育部重点实验室, 北京 100875)
    • 通讯作者: 赵葵
  • 收稿日期: 1900-01-01
  • 修回日期: 1900-01-01
  • 刊出日期: 2007-06-20

摘要: 在进行辐射致DNA损伤中, DNA浓度和剂量率是两个重要的因素。 利用γ射线在不同的剂量率下对不同浓度的质粒DNA pUC19水溶液进行了辐照， 利用凝胶电泳对辐照后的样品进行分析。 结果表明， 在同一剂量下被辐照的DNA样品， 随浓度的降低DNA损伤越来越严重； 通过分析软件和理论公式得到的每个DNA上的双链断裂数随着DNA浓度的变化呈现为非线性趋势。 As two factors， the changes of concentration of DNA and dose rate are of significance in the study of DNA damage induced by irradiation. The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated. The result of gel electrophoresis indicate that DNA molecules were damaged severely as the concentration of DNA decreasing. The number of double strand breakages per DNA were obtained by the software of Alpha Innotech and theoretical analysis. It shows that the numbers of double strand breakages per DNA presented nonlinear feature as the concentration of DNA decreasing, and the dose rate had little influence on DNA damage.

English Abstract

孔福全, 王潇, 倪嵋楠, 隋丽, 杨明建, 赵葵, #. γ射线诱导DNA损伤中DNA浓度和剂量率的影响[J]. 原子核物理评论, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
引用本文: 孔福全, 王潇, 倪嵋楠, 隋丽, 杨明建, 赵葵, #. γ射线诱导DNA损伤中DNA浓度和剂量率的影响[J]. 原子核物理评论, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
shu
KONG Fu-quan, WANG Xiao, NI Mei-nan, SUI Li, YANG Ming-jian, . Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray[J]. Nuclear Physics Review, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
Citation: KONG Fu-quan, WANG Xiao, NI Mei-nan, SUI Li, YANG Ming-jian, . Effect of Concentration of DNA and Dose Rate in DNA Damage Induced by γ Ray[J]. Nuclear Physics Review, 2007, 24(2): 103-107. doi: 10.11804/NuclPhysRev.24.02.103
shu

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