Effects of Cellcycle Progression of A549 Cells Induced by 12C6+ Ion Irradiation
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摘要: 选取对数生长期人肺癌细胞A549接受0—6.0 Gy 碳离子照射, 用克隆形成法检测细胞的存活率; 并于照射后12和24 h收集细胞, 用流式细胞术检测细胞周期各时相的细胞百分比, 观察不同剂量碳离子辐照对A549细胞周期进程的影响。 结果显示: 0—6.0 Gy 碳离子照射后细胞存活率显著下降; 照射后12 h细胞发生G0/G1期阻滞, 而照射后24 h, 1.0 Gy 照射组细胞在G0/G1期阻滞, 2.0—6.0 Gy 照射组细胞在G2/M期阻滞。 上述结果表明, 在A549细胞接受碳离子照射后的12 和24 h内, 1.0 Gy 照射可持续激活细胞G1期检查点, 而2.0—6.0 Gy 碳离子照射后其细胞周期进程是随时间变化的。 To investigate the effects of cellcycle progression of A549 cell induced by 12C6+ ion irradiation at different doses, the survival fractions of the A549 cells were determined by colonyforming assay; cell cycles were analyzed by FACS at 12 h or 24 h after irradiation. The results showed that the percentage of survival in the A549 cells decreased with irradiation doses. Compared with control group, the percentage of the cells in G0/G1 phase significantly increased at 12 h after irradiation with different doses of 12C6+ ions. However, at 24 h after irradiation the percentage of the cells in G0/G1 phase significantly increased with 1.0 Gy 12C6+ ions, while the cells showed increasing percentage in G2/M phase with 2.0, 4.0 or 6.0 Gy 12C6+ ions. The results suggested that G1 cell cycle checkpoint was activated in 12—24 h after irradiation with 1.0 Gy 12C6+ ions, but after irradiation with 2.0—6.0 Gy 12C6+ ions, the cellcycle progression of the A549 cells changed with time.Abstract: To investigate the effects of cellcycle progression of A549 cell induced by 12C6+ ion irradiation at different doses, the survival fractions of the A549 cells were determined by colonyforming assay; cell cycles were analyzed by FACS at 12 h or 24 h after irradiation. The results showed that the percentage of survival in the A549 cells decreased with irradiation doses. Compared with control group, the percentage of the cells in G0/G1 phase significantly increased at 12 h after irradiation with different doses of 12C6+ ions. However, at 24 h after irradiation the percentage of the cells in G0/G1 phase significantly increased with 1.0 Gy 12C6+ ions, while the cells showed increasing percentage in G2/M phase with 2.0, 4.0 or 6.0 Gy 12C6+ ions. The results suggested that G1 cell cycle checkpoint was activated in 12—24 h after irradiation with 1.0 Gy 12C6+ ions, but after irradiation with 2.0—6.0 Gy 12C6+ ions, the cellcycle progression of the A549 cells changed with time.
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Key words:
- 12C6+ ion irradiation /
- A549 cell /
- cell cycle /
- cell cycle checkpoint
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