关键词: 
碳离子辐照 /
人肺癌细胞A549 /
细胞周期 /
细胞周期检查点

摘要: 选取对数生长期人肺癌细胞A549接受0—6.0 Gy 碳离子照射， 用克隆形成法检测细胞的存活率； 并于照射后12和24 h收集细胞， 用流式细胞术检测细胞周期各时相的细胞百分比， 观察不同剂量碳离子辐照对A549细胞周期进程的影响。 结果显示： 0—6.0 Gy 碳离子照射后细胞存活率显著下降； 照射后12 h细胞发生G0/G1期阻滞， 而照射后24 h， 1.0 Gy 照射组细胞在G0/G1期阻滞， 2.0—6.0 Gy 照射组细胞在G2/M期阻滞。 上述结果表明， 在A549细胞接受碳离子照射后的12 和24 h内， 1.0 Gy 照射可持续激活细胞G1期检查点， 而2.0—6.0 Gy 碳离子照射后其细胞周期进程是随时间变化的。 To investigate the effects of cellcycle progression of A549 cell induced by ¹²C⁶⁺ ion irradiation at different doses, the survival fractions of the A549 cells were determined by colonyforming assay; cell cycles were analyzed by FACS at 12 h or 24 h after irradiation. The results showed that the percentage of survival in the A549 cells decreased with irradiation doses. Compared with control group， the percentage of the cells in G0/G1 phase significantly increased at 12 h after irradiation with different doses of ¹²C⁶⁺ ions. However， at 24 h after irradiation the percentage of the cells in G0/G1 phase significantly increased with 1.0 Gy ¹²C⁶⁺ ions， while the cells showed increasing percentage in G2/M phase with 2.0, 4.0 or 6.0 Gy ¹²C⁶⁺ ions. The results suggested that G1 cell cycle checkpoint was activated in 12—24 h after irradiation with 1.0 Gy ¹²C⁶⁺ ions， but after irradiation with 2.0—6.0 Gy ¹²C⁶⁺ ions， the cellcycle progression of the A549 cells changed with time.