重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

段昕; 张红; 邱嵘; 高清祥; 闵凤玲; 郝冀芳; 刘兵; 周清明; 李小达; 王燕玲; 周光明

doi:10.11804/NuclPhysRev.24.01.055

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重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

段昕, 张红, 邱嵘, 高清祥, 闵凤玲, 郝冀芳, 刘兵, 周清明, 李小达, 王燕玲, 周光明

文章导航 > 原子核物理评论 > 2007 > 24(1): 55-58

段昕, 张红, 邱嵘, 高清祥, 闵凤玲, 郝冀芳, 刘兵, 周清明, 李小达, 王燕玲, 周光明. 重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究[J]. 原子核物理评论, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055

引用本文:

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055

Citation:

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055

重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

doi: 10.11804/NuclPhysRev.24.01.055

1 中国科学院近代物理研究所, 甘肃兰州 730000;2 中国科学院研究生院, 北京 100049;3 兰州大学生命科学院, 甘肃兰州 730000

Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 School of Life Science, Lanzhou University, Lanzhou, 730000, China

摘要: 探讨¹²C⁶⁺ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMVGFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。采用不同剂量的¹²C⁶⁺ 重离子束辐射经AdCMVGFP 转染的B16细胞，利用流式细胞仪检测腺病毒的转染率。结果表明， ¹²C^6＋重离子束辐射能提高腺病毒对B16细胞的转染率，且具有量效关系。此外，先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.
- ¹²C⁶⁺离子束 /
- 重组腺病毒载体 /
- 转染效率 /
- 小鼠黑色素瘤细胞
Abstract: The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.
- ¹²C^6＋heavyion beam /
- AdCMVGFP /
- transfection efficiency /
- murine melanoma

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重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

doi: 10.11804/NuclPhysRev.24.01.055

1 中国科学院近代物理研究所, 甘肃兰州 730000;2 中国科学院研究生院, 北京 100049;3 兰州大学生命科学院, 甘肃兰州 730000

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2007-03-20

关键词:

¹²C⁶⁺离子束 /

重组腺病毒载体 /

转染效率 /

小鼠黑色素瘤细胞

摘要: 探讨¹²C⁶⁺ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMVGFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。采用不同剂量的¹²C⁶⁺ 重离子束辐射经AdCMVGFP 转染的B16细胞，利用流式细胞仪检测腺病毒的转染率。结果表明， ¹²C^6＋重离子束辐射能提高腺病毒对B16细胞的转染率，且具有量效关系。此外，先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of ¹²C⁶⁺ beam irradiation on AdCMVGFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMVGFP were irradiated by different doses of ¹²C⁶⁺ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that ¹²C⁶⁺ beam irradiation can improve tansfection efficiency of AdCMVGFP on murine melanoma cell B16 in a dosedependent manner. In addition， the tansfection efficiency in pretranfection plus irradiation group is higher than that in preirradiation plus tranfection group at the same dose irradiation dose.

English Abstract

引用本文:

Citation:

DUAN Xin, ZHANG Hong, QIU Rong, GAO Qing-xiang, MIN Feng-ling, HAO Ji-fang, LIU Bing, ZHOU Qing-ming, LI Xiao-da, WANG Yan-ling, ZHOU Guang-ming. Improvement of AdCMVGFP Gene Transfection Efficiency Induced by Heavy ion Beam Irradiation on Murine Melanoma cells[J]. Nuclear Physics Review, 2007, 24(1): 55-58. doi: 10.11804/NuclPhysRev.24.01.055