重离子诱导的质粒DNA双链断裂分布研究

王潇; 马秋峰; 靳根明; 李文建; 张红; 周利斌; 毛淑红; 邱嵘; 刘兵; 

doi:10.11804/NuclPhysRev.22.02.204

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重离子诱导的质粒DNA双链断裂分布研究

王潇, 马秋峰, 靳根明, 李文建, 张红, 周利斌, 毛淑红, 邱嵘, 刘兵, 

文章导航 > 原子核物理评论 > 2005 > 22(2): 204-207

王潇, 马秋峰, 靳根明, 李文建, 张红, 周利斌, 毛淑红, 邱嵘, 刘兵, . 重离子诱导的质粒DNA双链断裂分布研究[J]. 原子核物理评论, 2005, 22(2): 204-207. doi: 10.11804/NuclPhysRev.22.02.204

引用本文:

WANG Xiao, MA Qiu-feng, JIN Gen- ming, LI Wen-jain, ZHANG Hong, ZHOU Li-bin, MAO Shu-hong, QIU Rong, LIU Bing, . Study on Plasmid DNA Damage Induced by Low energy Neon Ion Irradiation[J]. Nuclear Physics Review, 2005, 22(2): 204-207. doi: 10.11804/NuclPhysRev.22.02.204

Citation:

WANG Xiao, MA Qiu-feng, JIN Gen- ming, LI Wen-jain, ZHANG Hong, ZHOU Li-bin, MAO Shu-hong, QIU Rong, LIU Bing, . Study on Plasmid DNA Damage Induced by Low energy Neon Ion Irradiation[J]. Nuclear Physics Review, 2005, 22(2): 204-207. doi: 10.11804/NuclPhysRev.22.02.204

重离子诱导的质粒DNA双链断裂分布研究

doi: 10.11804/NuclPhysRev.22.02.204

[1]中国科学院近代物理研究所,甘肃兰州730000[2]中国科学院近代物理研究所,甘肃兰州730000//中国科学院研究生院,北京100039

Study on Plasmid DNA Damage Induced by Low energy Neon Ion Irradiation

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;  2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China

摘要: 利用能量为7．2MeV／u氖离子束辐照体外质粒DNA：pUC18，采用恒场凝胶电泳结合多功能荧光成像系统研究了pUC18双链断裂片段的分布。证实了双链断裂片段分布的非随机性，结果还发现DNA断裂后片段的交联现象，而且交联片段的分布也是非随机的。
DNA is considered to be the most important and sensitive target in biological systems. In addition to the base damage, DNA strand breaks are the major lesion in the genome due to exposure to ionizing radiation. Mutation can be introduced to DNA as a result of enzymatic processing of DNA lesions or postirradiation replication. However, the mechanisms of radiationinduced mutations are not well clarified at the molecular level. To study the effect on the simple plasmid DNA of heavy ion is even predominant or more feasible. 
Plasmid pUC18 DNA was prepared and irradiated by neon beam (7.199 MeV/u). The fragment distributions were determined by quantifying the ethidium bromide fluorescence. It can be seen that the shape of the intensity distributions is vastly different for the used radiation Dose. The distribution produced shows an excess of fragments particularly in 3 000 and 10 000 Gy the size range between 20—40 kbp and 20—50 bp. This clustering of doublestranded fragments might be influenced by the higher order chromatin structure of genomic DNA. If so, DNA loop structures could correspond to the size range for which we observed DSB clustering. Further studies aim at elucidating the heterogeneity of DSB induction within the genome and investigate the influence of chromatin structure on the nonrandom fragment distribution.
- 重离子 /
- DNA双链断裂 /
- 非随机分布 /
- 质粒DNA
Abstract: DNA is considered to be the most important and sensitive target in biological systems. In addition to the base damage, DNA strand breaks are the major lesion in the genome due to exposure to ionizing radiation. Mutation can be introduced to DNA as a result of enzymatic processing of DNA lesions or postirradiation replication. However, the mechanisms of radiationinduced mutations are not well clarified at the molecular level. To study the effect on the simple plasmid DNA of heavy ion is even predominant or more feasible. 
Plasmid pUC18 DNA was prepared and irradiated by neon beam (7.199 MeV/u). The fragment distributions were determined by quantifying the ethidium bromide fluorescence. It can be seen that the shape of the intensity distributions is vastly different for the used radiation Dose. The distribution produced shows an excess of fragments particularly in 3 000 and 10 000 Gy the size range between 20—40 kbp and 20—50 bp. This clustering of doublestranded fragments might be influenced by the higher order chromatin structure of genomic DNA. If so, DNA loop structures could correspond to the size range for which we observed DSB clustering. Further studies aim at elucidating the heterogeneity of DSB induction within the genome and investigate the influence of chromatin structure on the nonrandom fragment distribution.
- heavy ions /
- DNA doublestrand break /
- nonrandom distribution /
- plasmid DNA

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重离子诱导的质粒DNA双链断裂分布研究

doi: 10.11804/NuclPhysRev.22.02.204

[1]中国科学院近代物理研究所,甘肃兰州730000[2]中国科学院近代物理研究所,甘肃兰州730000//中国科学院研究生院,北京100039

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2005-06-20

关键词:

重离子 /

DNA双链断裂 /

非随机分布 /

质粒DNA

摘要: 利用能量为7．2MeV／u氖离子束辐照体外质粒DNA：pUC18，采用恒场凝胶电泳结合多功能荧光成像系统研究了pUC18双链断裂片段的分布。证实了双链断裂片段分布的非随机性，结果还发现DNA断裂后片段的交联现象，而且交联片段的分布也是非随机的。
DNA is considered to be the most important and sensitive target in biological systems. In addition to the base damage, DNA strand breaks are the major lesion in the genome due to exposure to ionizing radiation. Mutation can be introduced to DNA as a result of enzymatic processing of DNA lesions or postirradiation replication. However, the mechanisms of radiationinduced mutations are not well clarified at the molecular level. To study the effect on the simple plasmid DNA of heavy ion is even predominant or more feasible. 
Plasmid pUC18 DNA was prepared and irradiated by neon beam (7.199 MeV/u). The fragment distributions were determined by quantifying the ethidium bromide fluorescence. It can be seen that the shape of the intensity distributions is vastly different for the used radiation Dose. The distribution produced shows an excess of fragments particularly in 3 000 and 10 000 Gy the size range between 20—40 kbp and 20—50 bp. This clustering of doublestranded fragments might be influenced by the higher order chromatin structure of genomic DNA. If so, DNA loop structures could correspond to the size range for which we observed DSB clustering. Further studies aim at elucidating the heterogeneity of DSB induction within the genome and investigate the influence of chromatin structure on the nonrandom fragment distribution.

English Abstract

Study on Plasmid DNA Damage Induced by Low energy Neon Ion Irradiation

1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;  2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2005-06-20

Keywords:

Abstract: DNA is considered to be the most important and sensitive target in biological systems. In addition to the base damage, DNA strand breaks are the major lesion in the genome due to exposure to ionizing radiation. Mutation can be introduced to DNA as a result of enzymatic processing of DNA lesions or postirradiation replication. However, the mechanisms of radiationinduced mutations are not well clarified at the molecular level. To study the effect on the simple plasmid DNA of heavy ion is even predominant or more feasible. 
Plasmid pUC18 DNA was prepared and irradiated by neon beam (7.199 MeV/u). The fragment distributions were determined by quantifying the ethidium bromide fluorescence. It can be seen that the shape of the intensity distributions is vastly different for the used radiation Dose. The distribution produced shows an excess of fragments particularly in 3 000 and 10 000 Gy the size range between 20—40 kbp and 20—50 bp. This clustering of doublestranded fragments might be influenced by the higher order chromatin structure of genomic DNA. If so, DNA loop structures could correspond to the size range for which we observed DSB clustering. Further studies aim at elucidating the heterogeneity of DSB induction within the genome and investigate the influence of chromatin structure on the nonrandom fragment distribution.

引用本文:

Citation:

WANG Xiao, MA Qiu-feng, JIN Gen- ming, LI Wen-jain, ZHANG Hong, ZHOU Li-bin, MAO Shu-hong, QIU Rong, LIU Bing, . Study on Plasmid DNA Damage Induced by Low energy Neon Ion Irradiation[J]. Nuclear Physics Review, 2005, 22(2): 204-207. doi: 10.11804/NuclPhysRev.22.02.204