TILLING技术的最新进展及其在辐射诱变育种中的应用前景

杜艳; <SUB>; </SUB> 余丽霞; <SUB>; </SUB> 刘青芳; 周利斌; #; 李文建; #

doi:10.11804/NuclPhysRev.28.03.343

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TILLING技术的最新进展及其在辐射诱变育种中的应用前景

杜艳, , 余丽霞, , 刘青芳, 周利斌, #, 李文建, #

文章导航 > 原子核物理评论 > 2011 > 28(3): 343-348

杜艳, , 余丽霞, , 刘青芳, 周利斌, #, 李文建, #. TILLING技术的最新进展及其在辐射诱变育种中的应用前景[J]. 原子核物理评论, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

引用本文:

杜艳, _, 余丽霞, _, 刘青芳, 周利斌, #, 李文建, #. TILLING技术的最新进展及其在辐射诱变育种中的应用前景[J]. 原子核物理评论, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

DU Yan, YU Li-xia, LIU Qing-fang, ZHOU Li-bin, #, LI Wen-jian, #. The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding[J]. Nuclear Physics Review, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

Citation:

DU Yan, YU Li-xia, LIU Qing-fang, ZHOU Li-bin, #, LI Wen-jian, #. The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding[J]. Nuclear Physics Review, 2011, 28(3): 343-348. doi: 10.11804/NuclPhysRev.28.03.343

TILLING技术的最新进展及其在辐射诱变育种中的应用前景

doi: 10.11804/NuclPhysRev.28.03.343

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 中国科学院研究生院, 北京 100049

The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding

Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

摘要: 定向诱导基因组局部突变技术(Targeting Induced Local Lesions IN Genomes, 简称TILLINC)是一种全新的、高通量和低成本反向遗传学研究方法。近年来, 随着突变筛选技术的革新, TILLING技术平台日趋多元化, 使得TILLING技术的操作更为简单﹑快速, 并广泛应用于作物育种研究领域。简要介绍了TILLING技术平台的最新发展动态, 并初步探讨了将辐射诱变处理与TILLING高通量筛选相结合在诱变育种中的应用前景。To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by ¹²C⁶⁺heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to ¹²C⁶⁺heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.
- 定向诱导基因组局部突变技术 /
- 高分辨率溶解曲线 /
- 辐射 /
- 诱变育种
Abstract: To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by ¹²C⁶⁺heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to ¹²C⁶⁺heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.
- The Latest Progress of TILLING Technique and Its Prospects in Irradiation Mutation Breeding /

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TILLING技术的最新进展及其在辐射诱变育种中的应用前景

doi: 10.11804/NuclPhysRev.28.03.343

1 中国科学院近代物理研究所, 甘肃兰州 730000; 2 中国科学院研究生院, 北京 100049

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2011-09-20

关键词:

定向诱导基因组局部突变技术 /

高分辨率溶解曲线 /

辐射 /

诱变育种

摘要: 定向诱导基因组局部突变技术(Targeting Induced Local Lesions IN Genomes, 简称TILLINC)是一种全新的、高通量和低成本反向遗传学研究方法。近年来, 随着突变筛选技术的革新, TILLING技术平台日趋多元化, 使得TILLING技术的操作更为简单﹑快速, 并广泛应用于作物育种研究领域。简要介绍了TILLING技术平台的最新发展动态, 并初步探讨了将辐射诱变处理与TILLING高通量筛选相结合在诱变育种中的应用前景。To investigate the M1 biological effects of heavy ions irradiation on Lycopersicon esculentum Mill., its seeds were irradiated by ¹²C⁶⁺heavy ions (80 MeV/u) with the dosages of 30, 60, 90, 120 and 160 Gy respectively . The results showed that with doses increased gradually, germination rate and seedling rate of Lycopersicon esculentum Mill. were decreased, and the latter was lower than the former, mainly due to the inhibition of root growth. The irradiation increased the content of MDA and proline evidently, showing irradiation could damage biomembrane, and also decreased the activities of POD and SOD with distinct inhibition pattern. However, the low dose and high dose irradiation promoted APX activity, illustrating APX was induced to protect irradiation injury. In brief, exposure to ¹²C⁶⁺heavy ions had obvious injury effects on the seeds of Lycopersicon esculentum Mill.. Heavy ions irradiation damaged biomembrane, inhibited activities of enzymes, and finally inhibited the growth of the first generation of these seeds.